A Type III CRISPR Ancillary Ribonuclease Degrades Its Cyclic Oligoadenylate Activator.
CRISPR
anti-viral signaling
cyclic oligoadenylate
ring nuclease, Thermus thermophilus
Journal
Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R
Informations de publication
Date de publication:
12 07 2019
12 07 2019
Historique:
received:
14
03
2019
revised:
22
04
2019
accepted:
29
04
2019
pubmed:
10
5
2019
medline:
6
6
2020
entrez:
10
5
2019
Statut:
ppublish
Résumé
Cyclic oligoadenylate (cOA) secondary messengers are generated by type III CRISPR systems in response to viral infection. cOA allosterically activates the CRISPR ancillary ribonucleases Csx1/Csm6, which degrade RNA non-specifically using a HEPN (Higher Eukaryotes and Prokaryotes, Nucleotide binding) active site. This provides effective immunity but can also lead to growth arrest in infected cells, necessitating a means to deactivate the ribonuclease once viral infection has been cleared. In the crenarchaea, dedicated ring nucleases degrade cA
Identifiants
pubmed: 31071326
pii: S0022-2836(19)30249-9
doi: 10.1016/j.jmb.2019.04.041
pmc: PMC6599890
pii:
doi:
Substances chimiques
Adenine Nucleotides
0
Oligoribonucleotides
0
2',5'-oligoadenylate
61172-40-5
RNA
63231-63-0
Ribonuclease III
EC 3.1.26.3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2894-2899Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S000313/1
Pays : United Kingdom
Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.
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