An Integrated Analysis of Metabolites, Peptides, and Inflammation Biomarkers for Assessment of Preanalytical Variability of Human Plasma.
blood
inflammation biomarkers
metabolites
peptides
plasma
preanalytical variability
sample quality
Journal
Journal of proteome research
ISSN: 1535-3907
Titre abrégé: J Proteome Res
Pays: United States
ID NLM: 101128775
Informations de publication
Date de publication:
07 06 2019
07 06 2019
Historique:
pubmed:
11
5
2019
medline:
28
5
2020
entrez:
11
5
2019
Statut:
ppublish
Résumé
Discrepancies in blood sample collection and processing could have a significant impact on levels of metabolites, peptides, and protein biomarkers of inflammation in the blood; thus, sample quality control is critical for successful biomarker identification and validation. In this study, we analyzed the effects of several preanalytical processing conditions, including different storage times and temperatures for blood or plasma samples and different centrifugation forces on the levels of metabolites, peptides, and inflammation biomarkers in human plasma samples using ethylenediaminetetraacetic acid (EDTA) as an anticoagulant. Temperature was found to be the major factor for metabolite variation, and both time and temperature were identified as major factors for peptide variation. For inflammation biomarkers, temperature played different roles depending on the sample type (blood or plasma). Low temperature affected inflammation biomarkers in blood, while room temperature impacted inflammation biomarkers in plasma.
Identifiants
pubmed: 31074987
doi: 10.1021/acs.jproteome.8b00903
pmc: PMC8477653
mid: NIHMS1741037
doi:
Substances chimiques
Biomarkers
0
Peptides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2411-2421Subventions
Organisme : Intramural FDA HHS
ID : FD999999
Pays : United States
Références
J Proteome Res. 2012 Dec 7;11(12):5748-62
pubmed: 23134551
Blood. 1996 Mar 15;87(6):2308-13
pubmed: 8630392
Biochimie. 1971;53(8):871-4
pubmed: 5148873
Clin Chim Acta. 1977 Oct 15;80(2):317-26
pubmed: 912905
Clin Chem. 1976 Oct;22(10):1702-4
pubmed: 975517
Prog Lipid Res. 2007 Mar;46(2):145-60
pubmed: 17459484
Environ Health Perspect. 2013 Apr;121(4):480-7
pubmed: 23384616
J Proteome Res. 2005 May-Jun;4(3):972-81
pubmed: 15952745
Bioinformatics. 2010 Apr 1;26(7):966-8
pubmed: 20147306
Clin Chem Lab Med. 2008;46(6):764-72
pubmed: 18601596
Biochem J. 1972 Aug;129(1):67-82
pubmed: 4675006
Acta Med Scand. 1963 Jul;174:123-7
pubmed: 14042462
Proc Soc Exp Biol Med. 1978 Sep;158(4):530-6
pubmed: 693470
Metabolomics. 2010 Mar;6(1):119-128
pubmed: 20339442
BMC Bioinformatics. 2012 Dec 06;13:325
pubmed: 23216942
J Chromatogr B Biomed Sci Appl. 1999 Jun 11;729(1-2):347-53
pubmed: 10410961
EBioMedicine. 2016 Oct;12:309-314
pubmed: 27596149
PLoS One. 2012;7(6):e38290
pubmed: 22701622
PLoS Comput Biol. 2017 Nov 3;13(11):e1005752
pubmed: 29099853
Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15049-54
pubmed: 23980179
Clin Chem. 2009 Jun;55(6):1218-22
pubmed: 19325012
Anasthesiol Intensivmed Notfallmed Schmerzther. 2001 Mar;36 Suppl 1:S45-50
pubmed: 11322111
J Proteome Res. 2008 Dec;7(12):5112-8
pubmed: 19367699
Biochim Biophys Acta. 2008 Sep;1781(9):496-502
pubmed: 18555808
Clin Chem. 2014 Feb;60(2):399-412
pubmed: 24305685
FEBS Lett. 1999 Jul 23;455(3):359-63
pubmed: 10437804