Tobacco exposure associated with oral microbiota oxygen utilization in the New York City Health and Nutrition Examination Study.
16S
Human microbiome
Microbiota
Oral health
RNA
Ribosomal
Smoking
Tobacco
Journal
Annals of epidemiology
ISSN: 1873-2585
Titre abrégé: Ann Epidemiol
Pays: United States
ID NLM: 9100013
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
25
07
2018
revised:
13
02
2019
accepted:
12
03
2019
pubmed:
12
5
2019
medline:
23
2
2020
entrez:
12
5
2019
Statut:
ppublish
Résumé
The effect of tobacco exposure on the oral microbiome has not been established. We performed amplicon sequencing of the 16S ribosomal RNA gene V4 variable region to estimate bacterial community characteristics in 259 oral rinse samples, selected based on self-reported smoking and serum cotinine levels, from the 2013-2014 New York City Health and Nutrition Examination Study. We identified differentially abundant operational taxonomic units (OTUs) by primary and secondhand tobacco exposure, and used "microbe set enrichment analysis" to assess shifts in microbial oxygen utilization. Cigarette smoking was associated with depletion of aerobic OTUs (Enrichment Score test statistic ES = -0.75, P = .002) with a minority (29%) of aerobic OTUs enriched in current smokers compared with never smokers. Consistent shifts in the microbiota were observed for current cigarette smokers as for nonsmokers with secondhand exposure as measured by serum cotinine levels. Differential abundance findings were similar in crude and adjusted analyses. Results support a plausible link between tobacco exposure and shifts in the oral microbiome at the population level through three lines of evidence: (1) a shift in microbiota oxygen utilization associated with primary tobacco smoke exposure; (2) consistency of abundance fold changes associated with current smoking and shifts along the gradient of secondhand smoke exposure among nonsmokers; and (3) consistency after adjusting for a priori hypothesized confounders.
Identifiants
pubmed: 31076212
pii: S1047-2797(18)30677-X
doi: 10.1016/j.annepidem.2019.03.005
pmc: PMC6548637
mid: NIHMS1525598
pii:
doi:
Substances chimiques
Biomarkers
0
RNA, Ribosomal, 16S
0
Tobacco Smoke Pollution
0
Cotinine
K5161X06LL
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18-25.e3Subventions
Organisme : NIAID NIH HHS
ID : R21 AI121784
Pays : United States
Organisme : NCI NIH HHS
ID : U24 CA180996
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
Références
Science. 1969 Dec 12;166(3911):1424-8
pubmed: 5350347
Cell Host Microbe. 2011 Oct 20;10(4):302-6
pubmed: 22018230
Nicotine Tob Res. 2016 Nov;18(11):2065-2074
pubmed: 27190401
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
J Community Health. 2015 Dec;40(6):1091-8
pubmed: 25929677
BMJ Open. 2014 Feb 11;4(2):e003736
pubmed: 24519874
Clin Prev Dent. 1989 Jan-Feb;11(1):3-7
pubmed: 2689047
PLoS One. 2010 Dec 20;5(12):e15216
pubmed: 21188149
Environ Microbiol. 2016 May;18(5):1403-14
pubmed: 26271760
Diabetes Metab J. 2012 Dec;36(6):399-403
pubmed: 23275932
J Clin Periodontol. 2013 Apr;40 Suppl 14:S181-94
pubmed: 23627328
Arthritis Res Ther. 2010;12(5):218
pubmed: 21062513
Int J Mol Sci. 2014 Dec 03;15(12):22279-95
pubmed: 25479074
Am J Respir Crit Care Med. 2008 Dec 1;178(11):1130-8
pubmed: 18723436
J Epidemiol Community Health. 2005 May;59(5):395-401
pubmed: 15831689
Int Dent J. 2009 Aug;59(4):197-209
pubmed: 19774803
J Periodontol. 1975 Feb;46(2):82-5
pubmed: 235017
Am J Respir Crit Care Med. 2013 May 15;187(10):1067-75
pubmed: 23491408
Am J Public Health. 1998 Jan;88(1):104-7
pubmed: 9584013
J Periodontol. 2013 Apr;84(4 Suppl):S135-52
pubmed: 23631574
Prev Med Rep. 2015 Jul 02;2:580-5
pubmed: 26844121
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 Apr;91(4):468-71
pubmed: 11312465
PLoS One. 2013 Apr 22;8(4):e61217
pubmed: 23630581
BMC Bioinformatics. 2016 Jan 20;17:45
pubmed: 26791995
J Periodontol. 2014 Oct;85(10):1450-7
pubmed: 24794687
Genome Biol. 2014;15(12):550
pubmed: 25516281
MMWR Morb Mortal Wkly Rep. 2015 Feb 6;64(4):103-8
pubmed: 25654612
Int J Epidemiol. 2015 Jun;44(3):735-43
pubmed: 26174518
JAMA. 1989 Jan 6;261(1):56-60
pubmed: 2908995
PLoS One. 2012;7(10):e46508
pubmed: 23094025
J Periodontal Res. 2006 Jun;41(3):184-8
pubmed: 16677286
Nucleic Acids Res. 2015 Apr 20;43(7):e47
pubmed: 25605792
Infect Immun. 2011 Nov;79(11):4730-8
pubmed: 21859855
Health Psychol. 1996 Nov;15(6):478-84
pubmed: 8973929
J Clin Periodontol. 2017 Mar;44(3):255-265
pubmed: 27978598
J Periodontol. 2001 Sep;72(9):1210-20
pubmed: 11577953
Nucleic Acids Res. 2007;35(21):7188-96
pubmed: 17947321
J Clin Periodontol. 2003 Nov;30(11):996-1002
pubmed: 14761123
Int J Environ Res Public Health. 2009 Jan;6(1):209-24
pubmed: 19440278
Clin Cancer Res. 2004 May 1;10(9):2922-7
pubmed: 15131026
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
ISME J. 2012 Jun;6(6):1176-85
pubmed: 22170420
Am J Public Health. 1987 Nov;77(11):1435-8
pubmed: 3661797
J Clin Periodontol. 2014 Nov;41(11):1037-47
pubmed: 25139209
J Periodontol. 2010 Dec;81(12):1708-24
pubmed: 20722533
ISME J. 2016 Oct;10(10):2435-46
pubmed: 27015003
Scand J Dent Res. 1984 Aug;92(4):315-8
pubmed: 6591368
BMC Bioinformatics. 2013 Jan 16;14:7
pubmed: 23323831
J Periodontol. 2000 Apr;71(4):550-4
pubmed: 10807117
Appl Environ Microbiol. 2005 Dec;71(12):8228-35
pubmed: 16332807
Metabolism. 1995 Feb;44(2 Suppl 2):18-22
pubmed: 7869932
PLoS One. 2016 Jan 21;11(1):e0146241
pubmed: 26795494
Nucleic Acids Res. 2012 Jan;40(Database issue):D115-22
pubmed: 22194640
Am J Epidemiol. 2009 Jan 15;169(2):236-48
pubmed: 19019851
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
J Dent. 2010 Feb;38(2):83-95
pubmed: 19895866
ISME J. 2015 Jan;9(1):268-72
pubmed: 25012901
Free Radic Biol Med. 2003 Feb 1;34(3):377-84
pubmed: 12543253
Nat Rev Immunol. 2009 May;9(5):377-84
pubmed: 19330016
J Multivar Anal. 2014 Jan 1;123:270-280
pubmed: 26028790
Handb Exp Pharmacol. 2009;(192):29-60
pubmed: 19184645