Molecular signatures identify immature mesenchymal progenitors in early mouse limb buds that respond differentially to morphogen signaling.
Cell sorting
Chondrogenesis
Mesenchymal progenitors
Mouse limb bud
Signaling
Transcriptome
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
28 05 2019
28 05 2019
Historique:
received:
30
10
2018
accepted:
01
05
2019
pubmed:
12
5
2019
medline:
17
3
2020
entrez:
12
5
2019
Statut:
epublish
Résumé
The key molecular interactions governing vertebrate limb bud development are a paradigm for studying the mechanisms controlling progenitor cell proliferation and specification during vertebrate organogenesis. However, little is known about the cellular heterogeneity of the mesenchymal progenitors in early limb buds that ultimately contribute to the chondrogenic condensations prefiguring the skeleton. We combined flow cytometric and transcriptome analyses to identify the molecular signatures of several distinct mesenchymal progenitor cell populations present in early mouse forelimb buds. In particular, jagged 1 (JAG1)-positive cells located in the posterior-distal mesenchyme were identified as the most immature limb bud mesenchymal progenitors (LMPs), which crucially depend on SHH and FGF signaling in culture. The analysis of gremlin 1 (
Identifiants
pubmed: 31076486
pii: dev.173328
doi: 10.1242/dev.173328
pmc: PMC6550019
pii:
doi:
Substances chimiques
Grem1 protein, mouse
0
Hedgehog Proteins
0
Intercellular Signaling Peptides and Proteins
0
Fibroblast Growth Factors
62031-54-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2019. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.
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