Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species.
Antineoplastic Agents, Phytogenic
/ chemistry
Caco-2 Cells
Cell Differentiation
/ drug effects
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Gene Expression Regulation, Neoplastic
/ drug effects
Hemidesmus
/ chemistry
Humans
Intestinal Absorption
Jurkat Cells
MicroRNAs
/ genetics
Plant Extracts
/ chemistry
Plant Roots
/ chemistry
Proteasome Endopeptidase Complex
/ genetics
Proteasome Inhibitors
/ chemistry
Reactive Oxygen Species
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
10 05 2019
10 05 2019
Historique:
received:
17
08
2018
accepted:
27
04
2019
entrez:
12
5
2019
pubmed:
12
5
2019
medline:
21
10
2020
Statut:
epublish
Résumé
Proteasome inhibition represents an important anticancer strategy. Here, we studied the mechanisms at the basis of the pro-apoptotic activity of the standardized decoction of Hemidesmus indicus, a plant evoking a complex anticancer activity, and explored its inhibition of proteasome activity in human leukemia cells. Additionally, we preliminary tested the cytotoxicity of some H. indicus's phytochemicals on leukemia cells and their intestinal absorption on a human intestinal epithelium model consisting of a monolayer of differentiated Caco2 cells. We observed a potent antileukemic effect for H. indicus, imputable to the modulation of different critical targets at protein and mRNA levels and the reduction of the 26S proteasome expression. We found that some phytomarkers of H. indicus decoction passed through the enterocyte monolayer. Overall, our study supports the pharmacological potential of H. indicus, which can represent an interesting botanical drug in the oncological area.
Identifiants
pubmed: 31076590
doi: 10.1038/s41598-019-43609-5
pii: 10.1038/s41598-019-43609-5
pmc: PMC6510901
doi:
Substances chimiques
Antineoplastic Agents, Phytogenic
0
MIRN15 microRNA, human
0
MIRN16 microRNA, human
0
MicroRNAs
0
Plant Extracts
0
Proteasome Inhibitors
0
Reactive Oxygen Species
0
Proteasome Endopeptidase Complex
EC 3.4.25.1
ATP dependent 26S protease
EC 3.4.99.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7199Références
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