N-terminal domain replacement changes an archaeal monoacylglycerol lipase into a triacylglycerol lipase.
Castor oil
Esterases
Lid domain
Lipases
TON-LPL
Thermococcus onnurineus (strain NA1)
Thermomyces lanuginosus (TLIP)
rc-TGL
Journal
Biotechnology for biofuels
ISSN: 1754-6834
Titre abrégé: Biotechnol Biofuels
Pays: England
ID NLM: 101316935
Informations de publication
Date de publication:
2019
2019
Historique:
received:
12
10
2018
accepted:
25
04
2019
entrez:
14
5
2019
pubmed:
14
5
2019
medline:
14
5
2019
Statut:
epublish
Résumé
Lipolytic enzymes of hyperthermophilic archaea generally prefer small carbon chain fatty acid esters (C TON-LPL and rc-TGL were cloned and overexpressed in Here, we have confirmed the predicted esterase activity of TON-LPL and also performed the lid engineering on TON-LPL which effectively expanded its substrate specificity from monoglycerides to triglycerides. This approach provides a way to engineer other hyperthermophilic esterases into industrially suitable lipases by employing N-terminal domain replacement. The immobilized preparation of rc-TGL has shown significant activity with castor oil and has a potential application in castor oil biorefinery to obtain value-added chemicals.
Sections du résumé
BACKGROUND
BACKGROUND
Lipolytic enzymes of hyperthermophilic archaea generally prefer small carbon chain fatty acid esters (C
RESULTS
RESULTS
TON-LPL and rc-TGL were cloned and overexpressed in
CONCLUSIONS
CONCLUSIONS
Here, we have confirmed the predicted esterase activity of TON-LPL and also performed the lid engineering on TON-LPL which effectively expanded its substrate specificity from monoglycerides to triglycerides. This approach provides a way to engineer other hyperthermophilic esterases into industrially suitable lipases by employing N-terminal domain replacement. The immobilized preparation of rc-TGL has shown significant activity with castor oil and has a potential application in castor oil biorefinery to obtain value-added chemicals.
Identifiants
pubmed: 31080517
doi: 10.1186/s13068-019-1452-5
pii: 1452
pmc: PMC6501381
doi:
Types de publication
Journal Article
Langues
eng
Pagination
110Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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