Neurogenesis Using P19 Embryonal Carcinoma Cells.

Animals Cell Differentiation / drug effects Embryonal Carcinoma Stem Cells / metabolism Image Processing, Computer-Assisted Mice Neurogenesis / drug effects Tretinoin / pharmacology

Journal

Journal of visualized experiments : JoVE
ISSN: 1940-087X
Titre abrégé: J Vis Exp
Pays: United States
ID NLM: 101313252

Informations de publication

Date de publication:
27 04 2019
Historique:
entrez: 14 5 2019
pubmed: 14 5 2019
medline: 21 3 2020
Statut: epublish

Résumé

The P19 cell line derived from a mouse embryo-derived teratocarcinoma has the ability to differentiate into the three germ layers. In the presence of retinoic acid (RA), the suspension cultured P19 cell line is induced to differentiate into neurons. This phenomenon is extensively investigated as a neurogenesis model in vitro. Therefore, the P19 cell line is very useful for molecular and cellular studies associated with neurogenesis. However, protocols for neuronal differentiation of P19 cell line described in the literature are very complex. The method developed in this study are simple and will play a part in elucidating the molecular mechanisms in neurodevelopmental abnormalities and neurodegenerative diseases.

Identifiants

DOI: 10.3791/58225 PMID: 31081818
pubmed: 31081818
doi: 10.3791/58225
doi:

Substances chimiques

Tretinoin 5688UTC01R

Types de publication

Journal Article Research Support, Non-U.S. Gov't Video-Audio Media

Langues

eng

Sous-ensembles de citation

IM

Auteurs

Paweł Leszczyński (P)

Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences.

Magdalena Śmiech (M)

Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences.

Aamir S Teeli (AS)

Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences.

Aleksandra Zołocińska (A)

Department of Regenerative Medicine, Maria Skłodowska-Curie Institute - Oncology Center.

Anna Słysz (A)

Department of Regenerative Medicine, Maria Skłodowska-Curie Institute - Oncology Center.

Zygmunt Pojda (Z)

Department of Regenerative Medicine, Maria Skłodowska-Curie Institute - Oncology Center.

Mariusz Pierzchała (M)

Department of Genomics and Biodiversities, Institute of Genetics and Animal Breeding, Polish Academy of Sciences.

Hiroaki Taniguchi (H)

Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences; h.taniguchi@ighz.pl.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Neurogenesis Using P19 Embryonal Carcinoma Cells.
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Neurogenesis Using P19 Embryonal Carcinoma Cells.
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches embryonnaires › Cellules souches de carcinome embryonnaire › Neurogenesis Using P19 Embryonal Carcinoma Cells.
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Neurogenesis Using P19 Embryonal Carcinoma Cells.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Neurogenesis Using P19 Embryonal Carcinoma Cells.
questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système nerveux › Neurogenèse › Neurogenesis Using P19 Embryonal Carcinoma Cells.
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Caroténoïdes › Rétinoïdes › Trétinoïne › Neurogenesis Using P19 Embryonal Carcinoma Cells.