Fibronectin-conjugated thermoresponsive nanobridges generate three dimensional human pluripotent stem cell cultures for differentiation towards the neural lineages.
3D cell culture
Neural progenitors
Thermoresponsive polymer
hESC
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
27
01
2019
revised:
31
03
2019
accepted:
15
04
2019
pubmed:
15
5
2019
medline:
22
4
2020
entrez:
15
5
2019
Statut:
ppublish
Résumé
Production of 3-dimensional neural progenitor cultures from human pluripotent stem cells offers the potential to generate large numbers of cells. We utilised our nanobridge system to generate 3D hPSC aggregates for differentiation towards the neural lineage, and investigate the ability to passage aggregates while maintaining cells at a stem/progenitor stage. Over 38 days, aggregate cultures exhibited upregulation and maintenance of neural-associated markers and demonstrated up to 10 fold increase in cell number. Aggregates undergoing neural induction in the presence or absence of nanobridges demonstrated no differences in marker expression, proliferation or viability. However, aggregates formed without nanobridges were statistically significantly fewer and smaller by passage 3. Organoids, cultured from aggregates, and treated with retinoic acid or rock inhibitor demonstrated terminal differentiation as assessed by immunohistochemistry. These data demonstrate that nanobridge 3D hPSC can differentiate to neural stem/progenitor cells, and be maintained at this stage through serial passaging and expansion.
Identifiants
pubmed: 31082678
pii: S1873-5061(19)30071-6
doi: 10.1016/j.scr.2019.101441
pii:
doi:
Substances chimiques
Fibronectins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101441Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.