Parvovirus B19 Uncoating Occurs in the Cytoplasm without Capsid Disassembly and It Is Facilitated by Depletion of Capsid-Associated Divalent Cations.
B19V
capsid stability
divalent cations
genome externalization
nuclear targeting
parvovirus
trafficking
uncoating
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
10 05 2019
10 05 2019
Historique:
received:
17
04
2019
revised:
07
05
2019
accepted:
09
05
2019
entrez:
15
5
2019
pubmed:
15
5
2019
medline:
25
7
2020
Statut:
epublish
Résumé
Human parvovirus B19 (B19V) traffics to the cell nucleus where it delivers the genome for replication. The intracellular compartment where uncoating takes place, the required capsid structural rearrangements and the cellular factors involved remain unknown. We explored conditions that trigger uncoating in vitro and found that prolonged exposure of capsids to chelating agents or to buffers with chelating properties induced a structural rearrangement at 4 °C resulting in capsids with lower density. These lighter particles remained intact but were unstable and short exposure to 37 °C or to a freeze-thaw cycle was sufficient to trigger DNA externalization without capsid disassembly. The rearrangement was not observed in the absence of chelating activity or in the presence of MgCl
Identifiants
pubmed: 31083301
pii: v11050430
doi: 10.3390/v11050430
pmc: PMC6563316
pii:
doi:
Substances chimiques
Capsid Proteins
0
Magnesium
I38ZP9992A
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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