A complex human gut microbiome cultured in an anaerobic intestine-on-a-chip.
Anaerobiosis
Bacteria
/ classification
Bacteroidetes
Biodiversity
Caco-2 Cells
Cell Culture Techniques
/ methods
Epithelial Cells
Feces
/ microbiology
Firmicutes
Gastrointestinal Microbiome
/ physiology
Host Microbial Interactions
/ physiology
Humans
Hypoxia
In Vitro Techniques
Intestinal Mucosa
/ microbiology
Lab-On-A-Chip Devices
Microbiota
/ physiology
Microfluidic Analytical Techniques
/ methods
Mucus
Oxygen
Journal
Nature biomedical engineering
ISSN: 2157-846X
Titre abrégé: Nat Biomed Eng
Pays: England
ID NLM: 101696896
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
11
09
2018
accepted:
04
04
2019
pubmed:
16
5
2019
medline:
10
3
2020
entrez:
16
5
2019
Statut:
ppublish
Résumé
The diverse bacterial populations that comprise the commensal microbiome of the human intestine play a central role in health and disease. A method that sustains complex microbial communities in direct contact with living human intestinal cells and their overlying mucus layer in vitro would thus enable the investigation of host-microbiome interactions. Here, we show the extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota, using a microfluidic intestine-on-a-chip that permits the control and real-time assessment of physiologically relevant oxygen gradients. When compared to aerobic coculture conditions, the establishment of a transluminal hypoxia gradient in the chip increased intestinal barrier function and sustained a physiologically relevant level of microbial diversity, consisting of over 200 unique operational taxonomic units from 11 different genera and an abundance of obligate anaerobic bacteria, with ratios of Firmicutes and Bacteroidetes similar to those observed in human faeces. The intestine-on-a-chip may serve as a discovery tool for the development of microbiome-related therapeutics, probiotics and nutraceuticals.
Identifiants
pubmed: 31086325
doi: 10.1038/s41551-019-0397-0
pii: 10.1038/s41551-019-0397-0
pmc: PMC6658209
mid: NIHMS1526407
doi:
Substances chimiques
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
520-531Subventions
Organisme : FDA HHS
ID : HHSF223201310079C
Pays : United States
Organisme : FDA HHS
ID : HHSF223201310079C
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK034854
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB020004
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : ErratumIn
Type : CommentIn
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