High-density multi-fiber photometry for studying large-scale brain circuit dynamics.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
14
08
2018
accepted:
28
03
2019
pubmed:
16
5
2019
medline:
10
7
2019
entrez:
16
5
2019
Statut:
ppublish
Résumé
Animal behavior originates from neuronal activity distributed across brain-wide networks. However, techniques available to assess large-scale neural dynamics in behaving animals remain limited. Here we present compact, chronically implantable, high-density arrays of optical fibers that enable multi-fiber photometry and optogenetic perturbations across many regions in the mammalian brain. In mice engaged in a texture discrimination task, we achieved simultaneous photometric calcium recordings from networks of 12-48 brain regions, including striatal, thalamic, hippocampal and cortical areas. Furthermore, we optically perturbed subsets of regions in VGAT-ChR2 mice by targeting specific fiber channels with a spatial light modulator. Perturbation of ventral thalamic nuclei caused distributed network modulation and behavioral deficits. Finally, we demonstrate multi-fiber photometry in freely moving animals, including simultaneous recordings from two mice during social interaction. High-density multi-fiber arrays are versatile tools for the investigation of large-scale brain dynamics during behavior.
Identifiants
pubmed: 31086339
doi: 10.1038/s41592-019-0400-4
pii: 10.1038/s41592-019-0400-4
doi:
Substances chimiques
Vesicular Inhibitory Amino Acid Transport Proteins
0
Viaat protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
553-560Références
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