Unraveling essential cellulosomal components of the (
Biotechnology
CBM
Cellulases
Cellulolytic bacteria
Cohesin
Dockerin
Enzymatic hydrolysis
Glycoside hydrolases
Scaffoldin
Journal
Biotechnology for biofuels
ISSN: 1754-6834
Titre abrégé: Biotechnol Biofuels
Pays: England
ID NLM: 101316935
Informations de publication
Date de publication:
2019
2019
Historique:
received:
18
02
2019
accepted:
20
04
2019
entrez:
16
5
2019
pubmed:
16
5
2019
medline:
16
5
2019
Statut:
epublish
Résumé
( The current proteome-wide work reveals patterns of protein expression of the various cellulosomal components, and explores the signature of differential expression upon growth of the bacterium on two major carbon sources-cellobiose and microcrystalline cellulose. Mass spectrometry analysis of the bacterial secretome revealed the expression of 24 scaffoldin structural units and 166 dockerin-bearing components (mainly enzymes), in addition to free enzymatic subunits. The dockerin-bearing components comprise cell-free and cell-bound cellulosomes for more efficient carbohydrate degradation. Various glycoside hydrolase (GH) family members were represented among 102 carbohydrate-degrading enzymes, including the omnipresent, most abundant GH48 exoglucanase. Specific cellulosomal components were found in different molecular-weight fractions associated with cell growth on different carbon sources. Overall, microcrystalline cellulose-derived cellulosomes showed markedly higher expression levels of the structural and enzymatic components, and exhibited the highest degradation activity on five different cellulosic and/or hemicellulosic carbohydrates. The cellulosomal activity of The current research demonstrates the involvement of key cellulosomal factors that participate in the mechanism of carbohydrate degradation by
Sections du résumé
BACKGROUND
BACKGROUND
(
RESULTS
RESULTS
The current proteome-wide work reveals patterns of protein expression of the various cellulosomal components, and explores the signature of differential expression upon growth of the bacterium on two major carbon sources-cellobiose and microcrystalline cellulose. Mass spectrometry analysis of the bacterial secretome revealed the expression of 24 scaffoldin structural units and 166 dockerin-bearing components (mainly enzymes), in addition to free enzymatic subunits. The dockerin-bearing components comprise cell-free and cell-bound cellulosomes for more efficient carbohydrate degradation. Various glycoside hydrolase (GH) family members were represented among 102 carbohydrate-degrading enzymes, including the omnipresent, most abundant GH48 exoglucanase. Specific cellulosomal components were found in different molecular-weight fractions associated with cell growth on different carbon sources. Overall, microcrystalline cellulose-derived cellulosomes showed markedly higher expression levels of the structural and enzymatic components, and exhibited the highest degradation activity on five different cellulosic and/or hemicellulosic carbohydrates. The cellulosomal activity of
CONCLUSIONS
CONCLUSIONS
The current research demonstrates the involvement of key cellulosomal factors that participate in the mechanism of carbohydrate degradation by
Identifiants
pubmed: 31086567
doi: 10.1186/s13068-019-1447-2
pii: 1447
pmc: PMC6507058
doi:
Types de publication
Journal Article
Langues
eng
Pagination
115Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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