The first seroepidemiological survey for Angiostrongylus vasorum in domestic dogs from Romania.
Angiostrongylus
/ isolation & purification
Animals
Antibodies, Helminth
/ blood
Antigens, Helminth
/ blood
Dog Diseases
/ epidemiology
Dogs
Enzyme-Linked Immunosorbent Assay
Female
Male
Pets
/ parasitology
Prevalence
Romania
/ epidemiology
Seroepidemiologic Studies
Strongylida Infections
/ epidemiology
Angiostrongylus vasorum
Antigen and antibody detection
Canine angiostrongylosis
Romania
Serology
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
14 May 2019
14 May 2019
Historique:
received:
07
01
2019
accepted:
03
05
2019
entrez:
16
5
2019
pubmed:
16
5
2019
medline:
25
6
2019
Statut:
epublish
Résumé
Angiostrongylus vasorum is a metastrongyloid nematode localized in the right heart and the pulmonary arteries of domestic dogs. The number of reports in Europe has recently increased, presumably as a consequence of a growing awareness among clinicians, animal owners and researchers, but also due to a growing incidence and territorial spread. So far, no studies have been conducted to assess the prevalence and distribution of A. vasorum in domestic dogs in Romania, and the awareness among veterinarians is limited or absent. The aim of the present study was to evaluate the countrywide seroprevalence of circulating antigens of A. vasorum and specific antibodies against A. vasorum in domestic dogs from Romania. Between November 2016 and July 2017, blood was sampled from a total of 1545 domestic dogs from 23 counties of Romania. Details about their gender, age, breed, housing, use and origin were collected. All serum samples were tested for the presence of A. vasorum circulating antigens (AG) using monoclonal and polyclonal antibodies in a sandwich ELISA. Additionally, a sandwich ELISA using A. vasorum adult somatic antigen purified by monoclonal antibodies was used for specific antibody (AB) detection. A total of 33 dogs (2.14%, 95% CI: 1.82-3.56%) were seropositive for A. vasorum antigen or antibodies against the parasite. Three dogs were positive for antigen only (0.19%, 95% CI: 0.07-0.57%) and 30 dogs (1.94%, 95% CI: 1.36-2.76%) were positive for antibodies only. No dog was positive for both tests. The overall prevalence (AB or AG) and the AB prevalence were significantly higher in pure breed dogs compared to mixed breeds and mongrel dogs (P < 0.05) and in shepherd dogs compared to other groups (P < 0.05). There was no significant difference between males and females, between urban and rural dogs, between dogs with unrestricted access and with restricted access to the environment, and between dogs living outdoors and indoors. Our data suggest that the disease is present in Romania in dogs, as it was previously demonstrated in foxes. However, so far, no clinical case has been reported in the country and this may be related to a low awareness among vets.
Sections du résumé
BACKGROUND
BACKGROUND
Angiostrongylus vasorum is a metastrongyloid nematode localized in the right heart and the pulmonary arteries of domestic dogs. The number of reports in Europe has recently increased, presumably as a consequence of a growing awareness among clinicians, animal owners and researchers, but also due to a growing incidence and territorial spread. So far, no studies have been conducted to assess the prevalence and distribution of A. vasorum in domestic dogs in Romania, and the awareness among veterinarians is limited or absent. The aim of the present study was to evaluate the countrywide seroprevalence of circulating antigens of A. vasorum and specific antibodies against A. vasorum in domestic dogs from Romania.
METHODS
METHODS
Between November 2016 and July 2017, blood was sampled from a total of 1545 domestic dogs from 23 counties of Romania. Details about their gender, age, breed, housing, use and origin were collected. All serum samples were tested for the presence of A. vasorum circulating antigens (AG) using monoclonal and polyclonal antibodies in a sandwich ELISA. Additionally, a sandwich ELISA using A. vasorum adult somatic antigen purified by monoclonal antibodies was used for specific antibody (AB) detection.
RESULTS
RESULTS
A total of 33 dogs (2.14%, 95% CI: 1.82-3.56%) were seropositive for A. vasorum antigen or antibodies against the parasite. Three dogs were positive for antigen only (0.19%, 95% CI: 0.07-0.57%) and 30 dogs (1.94%, 95% CI: 1.36-2.76%) were positive for antibodies only. No dog was positive for both tests. The overall prevalence (AB or AG) and the AB prevalence were significantly higher in pure breed dogs compared to mixed breeds and mongrel dogs (P < 0.05) and in shepherd dogs compared to other groups (P < 0.05). There was no significant difference between males and females, between urban and rural dogs, between dogs with unrestricted access and with restricted access to the environment, and between dogs living outdoors and indoors.
CONCLUSIONS
CONCLUSIONS
Our data suggest that the disease is present in Romania in dogs, as it was previously demonstrated in foxes. However, so far, no clinical case has been reported in the country and this may be related to a low awareness among vets.
Identifiants
pubmed: 31088513
doi: 10.1186/s13071-019-3481-0
pii: 10.1186/s13071-019-3481-0
pmc: PMC6515677
doi:
Substances chimiques
Antibodies, Helminth
0
Antigens, Helminth
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
224Références
Vet Parasitol. 2003 Aug 14;115(4):329-34
pubmed: 12944046
Parasitol Res. 2003 Dec;91(6):471-5
pubmed: 14557873
Trends Parasitol. 2005 Feb;21(2):49-51
pubmed: 15664523
Schweiz Arch Tierheilkd. 2005 Mar;147(3):121-7
pubmed: 15801623
J Wildl Dis. 2005 Oct;41(4):816-9
pubmed: 16456176
Vet J. 2009 Mar;179(3):348-59
pubmed: 18180185
Parasitol Int. 2009 Dec;58(4):406-10
pubmed: 19683596
Vet Parasitol. 2009 Dec 3;166(1-2):112-8
pubmed: 19717239
Vet Parasitol. 2009 Dec 23;166(3-4):326-32
pubmed: 19800738
J Vet Emerg Crit Care (San Antonio). 2010 Feb;20(1):98-109
pubmed: 20230439
Parasitol Res. 2010 Jun;107(1):135-40
pubmed: 20372926
Vet Parasitol. 2010 Sep 20;172(3-4):299-304
pubmed: 20570049
Vet Parasitol. 2010 Oct 29;173(3-4):255-61
pubmed: 20692767
Parasitol Res. 2011 Jul;109(1):63-9
pubmed: 21181189
Vet Parasitol. 2011 Jun 30;179(1-3):152-8
pubmed: 21333451
Vet Parasitol. 2012 Apr 30;185(2-4):216-24
pubmed: 22051072
Vet Parasitol. 2013 Feb 18;192(1-3):192-8
pubmed: 23182301
Parasitol Res. 2013 Aug;112 Suppl 1:109-17
pubmed: 23779223
Parasitology. 2013 Sep;140(11):1442-50
pubmed: 23965824
Acta Vet Hung. 2014 Mar;62(1):33-41
pubmed: 24334089
Parasit Vectors. 2014 Feb 18;7:72
pubmed: 24548539
Parasit Vectors. 2014 Aug 27;7:396
pubmed: 25164574
Vet Parasitol. 2015 Jan 15;207(1-2):81-4
pubmed: 25468671
Parasitology. 2015 Sep;142(10):1270-7
pubmed: 26036271
Parasitol Res. 2015 Aug;114 Suppl 1:S117-30
pubmed: 26152413
Parasitol Res. 2015 Aug;114 Suppl 1:S145-54
pubmed: 26152415
Vet Parasitol. 2015 Sep 15;212(3-4):219-26
pubmed: 26342624
Parasitol Res. 2016 Jul;115(7):2567-72
pubmed: 27000086
Vet Parasitol. 2017 Jan 15;233:14-19
pubmed: 28043382
J Vet Intern Med. 2017 Jul;31(4):1091-1099
pubmed: 28480552
Parasit Vectors. 2017 Jul 12;10(1):330
pubmed: 28701176
Parasitol Res. 2017 Aug;116(Suppl 1):31-40
pubmed: 28717947
Acta Parasitol. 2017 Dec 20;62(4):880-884
pubmed: 29035862
Acta Vet Scand. 2017 Dec 19;59(1):85
pubmed: 29258532
Int J Parasitol Parasites Wildl. 2017 Oct 21;6(3):349-353
pubmed: 29379713
C R Acad Sci Hebd Seances Acad Sci D. 1969 Dec 15;269(24):2377-80
pubmed: 4983280
Am J Vet Res. 1970 Jan;31(1):131-43
pubmed: 5414275