Use of trypan blue as an aqueous tracer dye to investigate hypotony where cyclodialysis cleft is suspected.


Journal

Clinical & experimental ophthalmology
ISSN: 1442-9071
Titre abrégé: Clin Exp Ophthalmol
Pays: Australia
ID NLM: 100896531

Informations de publication

Date de publication:
Sep 2019
Historique:
received: 22 12 2018
revised: 23 04 2019
accepted: 23 04 2019
pubmed: 16 5 2019
medline: 21 10 2020
entrez: 16 5 2019
Statut: ppublish

Résumé

A new method to help diagnose suspected cyclodialysis clefts. To study the use of trypan blue stained aqueous flow patterns in diagnosing causes of hypotony where cyclodialysis clefts were suspected. A case series in a tertiary care centre. Ten subjects presenting with persistent hypotony and retinal changes. Trypan blue was injected into the anterior chamber. The pattern of dye flow in the anterior chamber was categorized. Intraocular pressure prior to surgery was recorded. All seven subjects with cyclodialysis clefts had a preferential flow to the cleft region. Two hypotonous subjects post trabeculectomy had rapid (5 seconds) and extensive lymphatic staining (6 o'clock hours extent) without visible bleb formation. Preferential flow of dye to the limbus is a reliable sign of cyclodialysis cleft and helps localize cleft extent. A new cause of hypotony, "lymphatic overdrain," is identified.

Sections du résumé

IMPORTANCE OBJECTIVE
A new method to help diagnose suspected cyclodialysis clefts.
BACKGROUND BACKGROUND
To study the use of trypan blue stained aqueous flow patterns in diagnosing causes of hypotony where cyclodialysis clefts were suspected.
DESIGN METHODS
A case series in a tertiary care centre.
PARTICIPANTS METHODS
Ten subjects presenting with persistent hypotony and retinal changes.
METHODS METHODS
Trypan blue was injected into the anterior chamber.
MAIN OUTCOME MEASURES METHODS
The pattern of dye flow in the anterior chamber was categorized. Intraocular pressure prior to surgery was recorded.
RESULTS RESULTS
All seven subjects with cyclodialysis clefts had a preferential flow to the cleft region. Two hypotonous subjects post trabeculectomy had rapid (5 seconds) and extensive lymphatic staining (6 o'clock hours extent) without visible bleb formation.
CONCLUSIONS AND RELEVANCE CONCLUSIONS
Preferential flow of dye to the limbus is a reliable sign of cyclodialysis cleft and helps localize cleft extent. A new cause of hypotony, "lymphatic overdrain," is identified.

Identifiants

pubmed: 31090997
doi: 10.1111/ceo.13558
doi:

Substances chimiques

Coloring Agents 0
Trypan Blue I2ZWO3LS3M

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

904-908

Subventions

Organisme : National Health and Medical Research Council
ID : 107310
Organisme : National Health and Medical Research Council
ID : 102367

Informations de copyright

© 2019 Royal Australian and New Zealand College of Ophthalmologists.

Références

Küchle M, Naumann GO. Direct cyclopexy for traumatic cyclodialysis with persisting hypotony: report in 29 consecutive patients. Ophthalmology. 1995;102(2):322-333.
Yu D-Y, Morgan WH, Sun X, et al. The critical role of the conjunctiva in glaucoma filtration surgery. Prog Retin Eye Res. 2009;28(5):303-328.
Busacca A. Les Vaisseaux lymphatiques de la conjonctive bulbaire humaine etudies par la methode des injections vitales de bleutripan. Arch Ophthalmol. 1948;8:10.
Freitas-Neto CA, Costa RA, Kombo N, et al. Subconjunctival indocyanine green identifies lymphatic vessels. JAMA Ophthalmol. 2015;133(1):102-104.
Joo SH, Ko MK, Choe JK. Outflow of aqueous humor following cyclodialysis or ciliochoroidal detachment in rabbit. Korean J Ophthalmol. 1989;3(2):65-69.
Benedikt O. [Demonstration of aqueous outflow patterns of normal and glaucomatous human eyes through the injection of fluorescein solution in the anterior chamber (author's transl)]. Graefes Arch Clin Exp Ophthalmol. 1976;199(1):45-67.
Dada T, Muralidhar R, Sethi HS. Staining of filtering bleb with trypan blue during phacoemulsification. Eye (Lond). 2006;20(7):858-859.
Jhanji V, Chan E, Das S, Zhang H, Vajpayee RB. Trypan blue dye for anterior segment surgeries. Eye (Lond). 2011;25(9):1113-1120.
R Development Core Team. R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing; 2013.
Yu D-Y, Morgan W, Su EN, Cringle S. Clinical benefits for the monitoring and modulating of subconjunctival tissue following glaucoma filtration surgery. J Transl Sci. 2016;2. https://www.oatext.com/Clinical-benefits-for-the-monitoring-and-modulating-of-subconjunctival-tissue-following-glaucoma-filtration-surgery.php#Article_Info

Auteurs

Ying J Khoo (YJ)

Lions Eye Institute, University of Western Australia, Perth, Western Australia, Australia.

Aulia A H Abdullah (AAH)

Department of Ophthalmology, Faculty of Medicine, Brawijaya University Dr Saiful Anwar General Hospital, Malang, Indonesia.

Dao-Yi Yu (DY)

Lions Eye Institute, University of Western Australia, Perth, Western Australia, Australia.

William H Morgan (WH)

Lions Eye Institute, University of Western Australia, Perth, Western Australia, Australia.

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