Quantification of Cytoskeletal Dynamics in Time-Lapse Recordings.
catastrophe
growth speed
live-cell imaging
microtubule dynamics
microtubule-end labeling
quantitative fluorescence microscopy
rescue
shrink speed
Journal
Current protocols in plant biology
ISSN: 2379-8068
Titre abrégé: Curr Protoc Plant Biol
Pays: United States
ID NLM: 101685882
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
pubmed:
16
5
2019
medline:
27
11
2019
entrez:
16
5
2019
Statut:
ppublish
Résumé
The cytoskeleton is key to many essential processes in a plant cell, e.g., growth, division, and defense. Contrary to what "skeleton" implies, the cytoskeleton is highly dynamic, and is able to re-organize itself continuously. The advent of live-cell microscopy and the development of genetically encoded fluorophores enabled detailed observation of the organization and dynamics of the cytoskeleton. Despite the biological importance of the cytoskeletal dynamics, quantitative analyses remain laborious endeavors that only a handful of research teams regularly conduct. With this protocol, we provide a standardized step-by-step guide to analyze the dynamics of microtubules. We provide example data and code for post-processing in Fiji that enables researchers to modify and adapt the routine to their needs. More such tools are needed to quantitatively assess the cytoskeleton and thus to better understand cell biology. © 2019 by John Wiley & Sons, Inc.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e20091Informations de copyright
© 2019 John Wiley & Sons, Inc.