The Toxoplasma Vacuolar H
VAC
intracellular pH
lysosome
lytic cycle
micronemes
plant-like vacuole
proton transport
rhoptries
toxoplasma
vacuolar-H(+)-ATPase
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
14 05 2019
14 05 2019
Historique:
received:
06
06
2018
revised:
31
10
2018
accepted:
05
04
2019
entrez:
16
5
2019
pubmed:
16
5
2019
medline:
2
7
2020
Statut:
ppublish
Résumé
Vacuolar-proton ATPases (V-ATPases) are conserved complexes that couple the hydrolysis of ATP to the pumping of protons across membranes. V-ATPases are known to play diverse roles in cellular physiology. We studied the Toxoplasma gondii V-ATPase complex and discovered a dual role of the pump in protecting parasites against ionic stress and in the maturation of secretory proteins in endosomal-like compartments. Toxoplasma V-ATPase subunits localize to the plasma membrane and to acidic vesicles, and characterization of conditional mutants of the a1 subunit highlighted the functionality of the complex at both locations. Microneme and rhoptry proteins are required for invasion and modulation of host cells, and they traffic via endosome-like compartments in which proteolytic maturation occurs. We show that the V-ATPase supports the maturation of rhoptry and microneme proteins, and their maturases, during their traffic to their corresponding organelles. This work underscores a role for V-ATPases in regulating virulence pathways.
Identifiants
pubmed: 31091451
pii: S2211-1247(19)30503-0
doi: 10.1016/j.celrep.2019.04.038
pmc: PMC6760873
mid: NIHMS1529702
pii:
doi:
Substances chimiques
Protozoan Proteins
0
Vacuolar Proton-Translocating ATPases
EC 3.6.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2132-2146.e7Subventions
Organisme : NIAID NIH HHS
ID : R01 AI096836
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI100913
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI128356
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI060546
Pays : United States
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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