Hyperthermia Suppresses Post -
Animals
Apoptosis
/ radiation effects
Carcinoma
/ pathology
Caspase 3
/ genetics
Cell Line, Tumor
Cell Proliferation
/ genetics
Colonic Neoplasms
/ pathology
Cyclooxygenase 2
/ genetics
DNA Damage
/ genetics
Disease Models, Animal
Gene Expression Regulation, Neoplastic
/ genetics
Histones
/ genetics
Humans
Hyperthermia, Induced
Melanoma
/ pathology
Melanoma, Experimental
/ genetics
Mice
DNA damage
Hyperthermia
apoptosis
colon carcinoma
experimental cancer therapeutics
malignant melanoma
proliferation
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
01
04
2019
revised:
17
04
2019
accepted:
18
04
2019
entrez:
17
5
2019
pubmed:
17
5
2019
medline:
24
5
2019
Statut:
ppublish
Résumé
Several studies have highlighted hyperthermia's ability to enhance the effectiveness of radiation and chemotherapy in various in vitro and in vivo cancer models. In vivo murine models of malignant melanoma and colon carcinoma were utilized for demonstrating hyperthermia's therapeutic effectiveness by examining levels of caspase 3, COX-2 and phospho-H2A.X (Ser139) as endpoints of apoptosis, proliferation and DNA damage respectively. Hyperthermia induced in vitro cytotoxicity in malignant melanoma (B16-F10) and colon carcinoma (CT26) cell lines. In addition, it reduced post-in vitro proliferation and suppression of tumor growth by inducing the expression of caspase-3 and phospho-H2A.X (Ser139) while reducing the expression of COX-2 in both murine cancer models. Hyperthermia can exert therapeutic effectiveness against melanoma and colon carcinoma by inhibiting a number of critical cellular cascades including apoptosis, proliferation and DNA damage.
Sections du résumé
BACKGROUND
BACKGROUND
Several studies have highlighted hyperthermia's ability to enhance the effectiveness of radiation and chemotherapy in various in vitro and in vivo cancer models.
MATERIALS AND METHODS
METHODS
In vivo murine models of malignant melanoma and colon carcinoma were utilized for demonstrating hyperthermia's therapeutic effectiveness by examining levels of caspase 3, COX-2 and phospho-H2A.X (Ser139) as endpoints of apoptosis, proliferation and DNA damage respectively.
RESULTS
RESULTS
Hyperthermia induced in vitro cytotoxicity in malignant melanoma (B16-F10) and colon carcinoma (CT26) cell lines. In addition, it reduced post-in vitro proliferation and suppression of tumor growth by inducing the expression of caspase-3 and phospho-H2A.X (Ser139) while reducing the expression of COX-2 in both murine cancer models.
CONCLUSION
CONCLUSIONS
Hyperthermia can exert therapeutic effectiveness against melanoma and colon carcinoma by inhibiting a number of critical cellular cascades including apoptosis, proliferation and DNA damage.
Identifiants
pubmed: 31092422
pii: 39/5/2307
doi: 10.21873/anticanres.13347
doi:
Substances chimiques
H2AX protein, mouse
0
Histones
0
Cyclooxygenase 2
EC 1.14.99.1
Caspase 3
EC 3.4.22.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2307-2315Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.