MCM5 Expression Is Associated With the Grade of Malignancy and Ki-67 Antigen in LSCC.
Aged
Biomarkers, Tumor
/ genetics
Cell Cycle Proteins
/ genetics
Cell Line, Tumor
Cell Proliferation
/ genetics
Female
Gene Expression Regulation, Neoplastic
Humans
Keratinocytes
/ metabolism
Ki-67 Antigen
/ genetics
Laryngeal Neoplasms
/ genetics
Male
Middle Aged
Neoplasms
/ genetics
Squamous Cell Carcinoma of Head and Neck
/ genetics
Ki-67
MCM
laryngeal squamous cell cancer
minichromosome proteins
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
20
03
2019
revised:
11
04
2019
accepted:
12
04
2019
entrez:
17
5
2019
pubmed:
17
5
2019
medline:
24
5
2019
Statut:
ppublish
Résumé
The minichromosome maintenance proteins (MCMs) may be potential biomarkers of cancer cell proliferation. They are essential to initiate DNA replication. The aim of the study was to investigate the level of MCM5 expression in benign lesions (BLs) and laryngeal squamous cell cancer (LSCC). Immunohistochemical (IHC) analysis was carried out on 83 LSCCs and 10 BLs. Western-blot, immunofluorescence analysis (IF) and real-time PCR (RT-PCR) were performed using HEp-2 cancer cells and HaCaT keratinocytes. The expression of MCM5 was higher in LSCC than in the BLs (p<0.0001) and was higher in subsequent malignancies of LSCC. Positive correlations were demonstrated between the expression levels of MCM5 and the Ki-67 antigen. In vitro studies have confirmed that the expression of MCM5 is elevated in cancer cells. MCM5 protein may be used as a potential marker of cancer cell proliferation in LSCC.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
The minichromosome maintenance proteins (MCMs) may be potential biomarkers of cancer cell proliferation. They are essential to initiate DNA replication. The aim of the study was to investigate the level of MCM5 expression in benign lesions (BLs) and laryngeal squamous cell cancer (LSCC).
MATERIALS AND METHODS
METHODS
Immunohistochemical (IHC) analysis was carried out on 83 LSCCs and 10 BLs. Western-blot, immunofluorescence analysis (IF) and real-time PCR (RT-PCR) were performed using HEp-2 cancer cells and HaCaT keratinocytes.
RESULTS
RESULTS
The expression of MCM5 was higher in LSCC than in the BLs (p<0.0001) and was higher in subsequent malignancies of LSCC. Positive correlations were demonstrated between the expression levels of MCM5 and the Ki-67 antigen. In vitro studies have confirmed that the expression of MCM5 is elevated in cancer cells.
CONCLUSION
CONCLUSIONS
MCM5 protein may be used as a potential marker of cancer cell proliferation in LSCC.
Identifiants
pubmed: 31092424
pii: 39/5/2325
doi: 10.21873/anticanres.13349
doi:
Substances chimiques
Biomarkers, Tumor
0
Cell Cycle Proteins
0
Ki-67 Antigen
0
MCM5 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2325-2335Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.