Platelet Depletion/Transfusion as a Lethal Factor in a Colitis-associated Cancer Mouse Model.
Platelet depletion
colitis
mice
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
07
03
2019
revised:
30
03
2019
accepted:
03
04
2019
entrez:
17
5
2019
pubmed:
17
5
2019
medline:
23
5
2019
Statut:
ppublish
Résumé
TLR-4 Knock-out (KO) mice are protected from colitis-associated cancer in the established AOM/DSS mouse model. The aim of this study was to assess whether the TLR4 KO mice would still be protected from carcinogenesis after platelet depletion and transfusion with TLR4 wild-type platelets. Thirty-two female C57BL6 mice were divided into 6 groups. Among the three groups that received Azoxymethane/Dextran Sulfate Sodium (AOM/DSS), one group included TLR4KO mice, which were depleted of their platelets and were then transfused with platelets from TLR4 wild-type mice. The other two groups included wild-type and TLR-4KO mice that only received AOM/DSS. All 6 animals in the KO group that underwent platelet depletion/transfusion succumbed. Three of them died before the administration of DSS and three in the week following DSS administration. In contrast, mice in the other two groups experienced less weight loss and only 1 mouse died in each of them. Platelet depletion/transfusion was detrimental in TLR-4 transgenic mice that received AOM/DSS.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
TLR-4 Knock-out (KO) mice are protected from colitis-associated cancer in the established AOM/DSS mouse model. The aim of this study was to assess whether the TLR4 KO mice would still be protected from carcinogenesis after platelet depletion and transfusion with TLR4 wild-type platelets.
MATERIALS AND METHODS
METHODS
Thirty-two female C57BL6 mice were divided into 6 groups. Among the three groups that received Azoxymethane/Dextran Sulfate Sodium (AOM/DSS), one group included TLR4KO mice, which were depleted of their platelets and were then transfused with platelets from TLR4 wild-type mice. The other two groups included wild-type and TLR-4KO mice that only received AOM/DSS.
RESULTS
RESULTS
All 6 animals in the KO group that underwent platelet depletion/transfusion succumbed. Three of them died before the administration of DSS and three in the week following DSS administration. In contrast, mice in the other two groups experienced less weight loss and only 1 mouse died in each of them.
CONCLUSION
CONCLUSIONS
Platelet depletion/transfusion was detrimental in TLR-4 transgenic mice that received AOM/DSS.
Identifiants
pubmed: 31092437
pii: 39/5/2443
doi: 10.21873/anticanres.13362
doi:
Substances chimiques
Tlr4 protein, mouse
0
Toll-Like Receptor 4
0
Dextran Sulfate
9042-14-2
Azoxymethane
MO0N1J0SEN
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2443-2446Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.