Optimized Ki-67 staining in murine cells: a tool to determine cell proliferation.
Cell cycle
Cell proliferation
Flow cytometry
Hoechst staining
Immunofluorescent microscopy
Ki-67
Melanoma tumor
Mouse
Partial hepatectomy
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
02
01
2019
accepted:
02
05
2019
pubmed:
17
5
2019
medline:
15
2
2020
entrez:
17
5
2019
Statut:
ppublish
Résumé
The reliable analysis of the cell cycle status has become increasingly relevant for scientific and clinical work, especially for the determination of tumor cell growth. One established method to characterize the proliferation activity of cells is the analysis of the Ki-67 protein. Ki-67 is expressed in the nucleus during the whole cell cycle except for the G0 phase. Several different protocols exist for the examination of the Ki-67 protein in tissue and cell culture, but most of them are defined for human cells. For the analysis of the Ki-67 protein in murine tissue and cell culture there is a variety of protocols existing which recommend different fixation and permeabilization reagents or special kits. In this study, we established a reliable protocol for Ki-67 staining in murine cells and tissue based on PFA fixation, which can be used not only for flow cytometry but also for immunofluorescence microscopy analysis. We tested our protocol successfully with three different Ki-67 anti-mouse antibodies in cell culture, regenerating liver tissue and mouse melanoma tumor to demonstrate the general applicability.
Identifiants
pubmed: 31093875
doi: 10.1007/s11033-019-04851-2
pii: 10.1007/s11033-019-04851-2
doi:
Substances chimiques
Ki-67 Antigen
0
Mki67 protein, mouse
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4631-4643Subventions
Organisme : Bonfor (DE)
ID : eAntrag 2014-1-22
Organisme : Bonfor (DE)
ID : PSP: O-117.0055
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