A centrifuge tube reactor for the determination of bacterial methane oxidation enrichment factors without influence of diffusion related isotope fractionation.
Isotope fractionation
Methane oxidation
Stable isotope analysis
Journal
The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500
Informations de publication
Date de publication:
01 Apr 2019
01 Apr 2019
Historique:
received:
12
11
2018
revised:
17
12
2018
accepted:
18
12
2018
entrez:
18
5
2019
pubmed:
18
5
2019
medline:
15
6
2019
Statut:
ppublish
Résumé
Biotransformation of methane at landfill sites can be estimated by applying compound specific stable isotope analysis of methane from the anaerobic and the cover layer surface zone. Next to these two input parameters, merely the knowledge of the carbon isotopic fractionation of the bacterial methane oxidation in terms of the enrichment factor (ε) is required. However, many factors and conditions have been described to affect ε. These include temperature, the applied landfill cover, the type of expressed methane monooxygenase (MMO), and cell density. In this work we investigated the microbial methane oxidation with respect to temperature and type of methanotrophic enrichment culture. A newly designed setup was used to overcome potential CH
Identifiants
pubmed: 31096348
pii: S0048-9697(18)35156-8
doi: 10.1016/j.scitotenv.2018.12.283
pii:
doi:
Substances chimiques
Oxygenases
EC 1.13.-
methane monooxygenase
EC 1.14.13.25
Methane
OP0UW79H66
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1382-1386Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.