A Nucleus-Targeting DNA Aptamer for Dead Cell Indication.

Animals Apoptosis Aptamers, Nucleotide / chemistry Cell Death Cell Line, Tumor Cell Nucleus / chemistry DNA / chemistry Humans Mice Neoplasms / classification Nucleoproteins / analysis Oligodeoxyribonucleotides / chemistry SELEX Aptamer Technique
aptamer cell analysis dead cells nuclear probe tissue imaging

Journal

ACS sensors
ISSN: 2379-3694
Titre abrégé: ACS Sens
Pays: United States
ID NLM: 101669031

Informations de publication

Date de publication:
28 06 2019
Historique:
pubmed: 18 5 2019
medline: 25 4 2020
entrez: 18 5 2019
Statut: ppublish

Résumé

Dead cells always accompany with live cells in vivo and in cell culture. It is important to distinguish dead cells from live cells in various biological studies. Currently, the probes for dead cells are mainly nucleic acid-intercalators, most of which have low affinity and potential toxicity to live cells. In this work, we report a novel aptameric probe (Ch4-1) for the first time, which binds cell nuclei with high affinity (apparent K

Identifiants

DOI: 10.1021/acssensors.9b00342 PMID: 31099246
pubmed: 31099246
doi: 10.1021/acssensors.9b00342
doi:

Substances chimiques

Aptamers, Nucleotide 0
Nucleoproteins 0
Oligodeoxyribonucleotides 0
DNA 9007-49-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1612-1618

Auteurs

Luyao Shen (L)

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences , Institute of Chemistry, Chinese Academy of Sciences , Beijing , 100190 , China.
University of Chinese Academy of Sciences , Beijing , 100049 , China.
ORCID: 0000-0002-3231-4878

Tao Bing (T)

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences , Institute of Chemistry, Chinese Academy of Sciences , Beijing , 100190 , China.
University of Chinese Academy of Sciences , Beijing , 100049 , China.
ORCID: 0000-0002-5121-053X

Nan Zhang (N)

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences , Institute of Chemistry, Chinese Academy of Sciences , Beijing , 100190 , China.
University of Chinese Academy of Sciences , Beijing , 100049 , China.

LinLin Wang (L)

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences , Institute of Chemistry, Chinese Academy of Sciences , Beijing , 100190 , China.
University of Chinese Academy of Sciences , Beijing , 100049 , China.

Junyan Wang (J)

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences , Institute of Chemistry, Chinese Academy of Sciences , Beijing , 100190 , China.
University of Chinese Academy of Sciences , Beijing , 100049 , China.

Xiangjun Liu (X)

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences , Institute of Chemistry, Chinese Academy of Sciences , Beijing , 100190 , China.
University of Chinese Academy of Sciences , Beijing , 100049 , China.
ORCID: 0000-0002-0119-2134

Dihua Shangguan (D)

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences , Institute of Chemistry, Chinese Academy of Sciences , Beijing , 100190 , China.
University of Chinese Academy of Sciences , Beijing , 100049 , China.
ORCID: 0000-0002-5746-803X

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Noyau de la cellule › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Tumeurs › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Oligodésoxyribonucléotides › A Nucleus-Targeting DNA Aptamer for Dead Cell...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Techniques de chimie combinatoire › Technique SELEX › A Nucleus-Targeting DNA Aptamer for Dead Cell...