Genomic landscape analyses of reprogrammed cells using integrative and non-integrative methods reveal variable cancer-associated alterations.
CGH array
genetic instability
iPSCs
Journal
Oncotarget
ISSN: 1949-2553
Titre abrégé: Oncotarget
Pays: United States
ID NLM: 101532965
Informations de publication
Date de publication:
12 Apr 2019
12 Apr 2019
Historique:
received:
25
09
2018
accepted:
23
03
2019
entrez:
21
5
2019
pubmed:
21
5
2019
medline:
21
5
2019
Statut:
epublish
Résumé
Recent development of cell reprogramming technologies brought a major hope for future cell therapy applications by the use of these cells or their derivatives. For this purpose, one of the major requirements is the absence of genomic alterations generating a risk of cell transformation. Here we analyzed by microarray-based comparative genomic hybridization human iPSC generated by two non-integrative and one integrative method at pluripotent stage as well as in corresponding teratomas. We show that all iPSC lines exhibit copy number variations (CNV) of several genes deregulated in oncogenesis. These cancer-associated genomic alterations were more pronounced in virally programmed hiPSCs and their derivative teratoma as compared to those found in iPSC generated by mRNA-mediated reprogramming. Bioinformatics analysis showed the involvement of these genes in human leukemia and carcinoma. We conclude that genetic screening should become a standard procedure to ensure that hiPSCs are free from cancer-associated genomic alterations before clinical use.
Identifiants
pubmed: 31105870
doi: 10.18632/oncotarget.26857
pii: 26857
pmc: PMC6505633
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2693-2708Déclaration de conflit d'intérêts
CONFLICTS OF INTEREST There are no conflicts of interest to disclose.
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