Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin.

Animals Bleomycin / pharmacology Disease Models, Animal Female Lung Injury / chemically induced Mesenchymal Stem Cell Transplantation / methods Mesenchymal Stem Cells Mice Mice, Inbred C57BL Pulmonary Fibrosis / chemically induced Trachea Umbilical Cord / cytology

Journal

Journal of visualized experiments : JoVE
ISSN: 1940-087X
Titre abrégé: J Vis Exp
Pays: United States
ID NLM: 101313252

Informations de publication

Date de publication:
30 04 2019
Historique:
entrez: 21 5 2019
pubmed: 21 5 2019
medline: 19 3 2020
Statut: epublish

Résumé

Pulmonary fibrosis is a hallmark of several human lung diseases with a different etiology. Since current therapies are rather limited, mouse models continue to be an essential tool for developing new antifibrotic strategies. Here we provide an effective method to investigate in vivo antifibrotic activity of human mesenchymal stromal cells obtained from whole umbilical cord (hUC-MSC) in attenuating bleomycin-induced lung injury. C57BL/6 mice receive a single endotracheal injection of bleomycin (1.5 U/kg body weight) followed by a double infusion of hUC-MSC (2.5 x 10

Identifiants

DOI: 10.3791/58922 PMID: 31107440
pubmed: 31107440
doi: 10.3791/58922
doi:

Substances chimiques

Bleomycin 11056-06-7

Types de publication

Journal Article Research Support, Non-U.S. Gov't Video-Audio Media

Langues

eng

Sous-ensembles de citation

IM

Auteurs

Fiorenza Orlando (F)

Servizio di Allevamento e Sperimentazione Animale, Polo ScientificoTecnologico, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)-Istituto Nazionale Ricovero e Cura Anziani (INRCA).

Chiara Paolini (C)

Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche.

Silvia Agarbati (S)

Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche.

Cecilia Tonnini (C)

Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche.

Antonella Grieco (A)

Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche.

Chiara Capelli (C)

UOS Centro di Terapia Cellulare "G. Lanzani", Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII.

Martino Introna (M)

UOS Centro di Terapia Cellulare "G. Lanzani", Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII.

Mauro Provinciali (M)

Servizio di Allevamento e Sperimentazione Animale, Polo ScientificoTecnologico, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)-Istituto Nazionale Ricovero e Cura Anziani (INRCA).

Armando Gabrielli (A)

Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche.

Gianluca Moroncini (G)

Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche; g.moroncini@univpm.it.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Induction of Mouse Lung Injury by Endotracheal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Glycopeptides › Bléomycine › Induction of Mouse Lung Injury by Endotracheal...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Induction of Mouse Lung Injury by Endotracheal...
questionsmedicales.fr › Plaies et blessures › Blessures du thorax › Lésion pulmonaire › Induction of Mouse Lung Injury by Endotracheal...
questionsmedicales.fr › Procédures de chirurgie opératoire › Transplantation › Transplantation cellulaire › Transplantation de cellules souches › Transplantation de cellules souches mésenchymateuses › Induction of Mouse Lung Injury by Endotracheal...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Induction of Mouse Lung Injury by Endotracheal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Induction of Mouse Lung Injury by Endotracheal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › Induction of Mouse Lung Injury by Endotracheal...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Fibrose › Fibrose pulmonaire › Induction of Mouse Lung Injury by Endotracheal...
questionsmedicales.fr › Appareil respiratoire › Trachée › Induction of Mouse Lung Injury by Endotracheal...
questionsmedicales.fr › Structures de l'embryon › Foetus › Cordon ombilical › Induction of Mouse Lung Injury by Endotracheal...