The relationship between the presence of antibodies and direct detection of Toxoplasma gondii in slaughtered calves and cattle in four European countries.
Animals
Antibodies, Protozoan
/ blood
Cattle
Cattle Diseases
/ diagnosis
Diagnostic Tests, Routine
/ methods
Diaphragm
/ parasitology
Europe
Immunoassay
/ methods
Immunoglobulin G
/ blood
Liver
/ parasitology
Molecular Diagnostic Techniques
/ methods
Sensitivity and Specificity
Serum
/ immunology
Toxoplasma
/ isolation & purification
Toxoplasmosis, Animal
/ diagnosis
Cattle
Detection
Mouse bioassay
PCR
Serology
Toxoplasma gondii
Journal
International journal for parasitology
ISSN: 1879-0135
Titre abrégé: Int J Parasitol
Pays: England
ID NLM: 0314024
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
28
09
2018
revised:
24
01
2019
accepted:
27
01
2019
pubmed:
21
5
2019
medline:
19
3
2020
entrez:
21
5
2019
Statut:
ppublish
Résumé
In cattle, antibodies to Toxoplasma gondii infection are frequently detected, but evidence for the presence of T. gondii tissue cysts in cattle is limited. To study the concordance between the presence of anti-T. gondii IgG and viable tissue cysts of T. gondii in cattle, serum, liver and diaphragm samples of 167 veal calves and 235 adult cattle were collected in Italy, the Netherlands, Romania and the United Kingdom. Serum samples were tested for anti-T. gondii IgG by the modified agglutination test and p30 immunoblot. Samples from liver were analyzed by mouse bioassay and PCR after trypsin digestion. In addition, all diaphragms of cattle that had tested T. gondii-positive (either in bioassay, by PCR on trypsin-digested liver or serologically by MAT) and a selection of diaphragms from cattle that had tested negative were analyzed by magnetic capture quantitative PCR (MC-PCR). Overall, 13 animals were considered positive by a direct detection method: seven out of 151 (4.6%) by MC-PCR and six out of 385 (1.6%) by bioassay, indicating the presence of viable parasites. As cattle that tested positive in the bioassay tested negative by MC-PCR and vice-versa, these results demonstrate a lack of concordance between the presence of viable parasites in liver and the detection of T. gondii DNA in diaphragm. In addition, the probability to detect T. gondii parasites or DNA in seropositive and seronegative cattle was comparable, demonstrating that serological testing by MAT or p30 immunoblot does not provide information about the presence of T. gondii parasites or DNA in cattle and therefore is not a reliable indicator of the risk for consumers.
Identifiants
pubmed: 31108097
pii: S0020-7519(19)30129-8
doi: 10.1016/j.ijpara.2019.01.005
pii:
doi:
Substances chimiques
Antibodies, Protozoan
0
Immunoglobulin G
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
515-522Informations de copyright
Copyright © 2019 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.