A review on methodologies for extraction, identification and quantification of allergenic proteins in prawns.
Aptamer
Extraction
Prawn allergy
Quantification
Tropomyosin
Journal
Food research international (Ottawa, Ont.)
ISSN: 1873-7145
Titre abrégé: Food Res Int
Pays: Canada
ID NLM: 9210143
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
18
12
2018
revised:
13
03
2019
accepted:
17
03
2019
entrez:
22
5
2019
pubmed:
22
5
2019
medline:
22
9
2020
Statut:
ppublish
Résumé
Prawn allergy is one of the most common food-borne allergies and current prevention is by avoidance. This review paper summarised different methodologies for the extraction, identification and quantification of prawn protein allergens, reported in various research studies. Following extraction, allergenic components have been analysed using well-established methodologies, such as SDS-PAGE, Immunoblotting, ELISA, CD Spectroscopy, HPLC, DBPCFC, SPT etc. Moreover, the preference towards Aptamer-based technique for allergenicity analysis has also been highlighted in this review paper. The summary of these methodologies will provide a reference platform for present and future research directions.
Identifiants
pubmed: 31108753
pii: S0963-9969(19)30191-7
doi: 10.1016/j.foodres.2019.03.040
pii:
doi:
Substances chimiques
Allergens
0
Arthropod Proteins
0
Tropomyosin
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
307-318Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.