Determination of Kanamycin by High Performance Liquid Chromatography.
HPLC
Kanamycin
different detectors
food contamination
sample pre-treatment
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
17 May 2019
17 May 2019
Historique:
received:
01
04
2019
revised:
15
05
2019
accepted:
15
05
2019
entrez:
22
5
2019
pubmed:
22
5
2019
medline:
16
11
2019
Statut:
epublish
Résumé
Kanamycin is an aminoglycoside antibiotic widely used in treating animal diseases caused by Gram-negative and Gram-positive infections. Kanamycin has a relatively narrow therapeutic index, and can accumulate in the human body through the food chain. The abuse of kanamycin can have serious side-effects. Therefore, it was necessary to develop a sensitive and selective analysis method to detect kanamycin residue in food to ensure public health. There are many analytical methods to determine kanamycin concentration, among which high performance liquid chromatography (HPLC) is a common and practical tool. This paper presents a review of the application of HPLC analysis of kanamycin in different sample matrices. The different detectors coupled with HPLC, including Ultraviolet (UV)/Fluorescence, Evaporative Light Scattering Detector (ELSD)/Pulsed Electrochemical Detection (PED), and Mass Spectrometry, are discussed. Meanwhile, the strengths and weaknesses of each method are compared. The pre-treatment methods of food samples, including protein precipitation, liquid-liquid extraction (LLE), and solid-phase extraction (SPE) are also summarized in this paper.
Identifiants
pubmed: 31108895
pii: molecules24101902
doi: 10.3390/molecules24101902
pmc: PMC6572613
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Kanamycin
59-01-8
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 31601536
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