Effect of Geometrical Structure, Drying, and Synthetic Method on Aminated Chitosan-Coated Magnetic Nanoparticles Utility for HSA Effective Immobilization.
Adsorption
Amination
Chitosan
/ chemistry
Coated Materials, Biocompatible
Humans
Immobilized Proteins
/ chemistry
Magnetite Nanoparticles
/ chemistry
Models, Molecular
Models, Theoretical
Molecular Conformation
Polymers
/ chemistry
Serum Albumin, Human
/ chemistry
Solvents
Spectroscopy, Fourier Transform Infrared
HSA
aminated chitosan
magnetic nanoparticles
molecular dynamics simulations
protein immobilization
solvent-free amination
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
18 May 2019
18 May 2019
Historique:
received:
30
04
2019
revised:
14
05
2019
accepted:
17
05
2019
entrez:
22
5
2019
pubmed:
22
5
2019
medline:
15
11
2019
Statut:
epublish
Résumé
Human serum albumin (HSA) is one of the most frequently immobilized proteins on the surface of carriers, including magnetic nanoparticles. This is because the drug-HSA interaction study is one of the basic pharmacokinetic parameters determined for drugs. In spite of many works describing the immobilization of HSA and the binding of active substances, research describing the influence of the used support on the effectiveness of immobilization is missing. There are also no reports about the effect of the support drying method on the effectiveness of protein immobilization. This paper examines the effect of both the method of functionalizing the polymer coating covering magnetic nanoparticles (MNPs), and the drying methods for the immobilization of HSA. Albumin was immobilized on three types of aminated chitosan-coated nanoparticles with a different content of amino groups long distanced from the surface Fe
Identifiants
pubmed: 31109114
pii: molecules24101925
doi: 10.3390/molecules24101925
pmc: PMC6572529
pii:
doi:
Substances chimiques
Coated Materials, Biocompatible
0
Immobilized Proteins
0
Magnetite Nanoparticles
0
Polymers
0
Solvents
0
Chitosan
9012-76-4
Serum Albumin, Human
ZIF514RVZR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Narodowe Centrum Nauki
ID : 2014/15/D/NZ7/01805
Organisme : Narodowe Centrum Nauki
ID : 2014/13/B/ST8/04342
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10037-41
pubmed: 11517324
Chem Rev. 1997 Oct 1;97(6):2373-2420
pubmed: 11848903
Angew Chem Int Ed Engl. 2004 Jan 3;43(3):357-60
pubmed: 14705097
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W665-7
pubmed: 15215472
J Comput Chem. 2005 Dec;26(16):1781-802
pubmed: 16222654
Biomacromolecules. 2010 Nov 8;11(11):3196-207
pubmed: 20961133
Biopolymers. 2011 Jul;95(7):448-60
pubmed: 21328576
J Chem Theory Comput. 2011 Oct 11;7(10):3162-3180
pubmed: 22125473
J Colloid Interface Sci. 2012 Mar 1;369(1):96-102
pubmed: 22209576
J Chem Inf Model. 2012 Dec 21;52(12):3155-68
pubmed: 23145473
J Chem Inf Model. 2012 Dec 21;52(12):3144-54
pubmed: 23146088
Cancers (Basel). 2011 Jan 21;3(1):428-45
pubmed: 24212623
Int J Mol Sci. 2014 Feb 27;15(3):3580-95
pubmed: 24583848
Mater Sci Eng C Mater Biol Appl. 2014 Sep;42:546-52
pubmed: 25063152
Molecules. 2014 Aug 04;19(8):11465-86
pubmed: 25093986
Int J Biol Macromol. 2015 Feb;73:76-83
pubmed: 25445689
J Chem Theory Comput. 2008 Dec 9;4(12):2141-9
pubmed: 26620485
Int J Biol Macromol. 2016 Mar;84:281-8
pubmed: 26706842
Nanomaterials (Basel). 2014 Apr 02;4(2):222-241
pubmed: 28344220
Protein Sci. 2018 Jan;27(1):112-128
pubmed: 28836357
Nanomaterials (Basel). 2017 Aug 29;7(9):null
pubmed: 28850089
Nanomaterials (Basel). 2018 Mar 16;8(3):null
pubmed: 29547559
Materials (Basel). 2018 Jul 06;11(7):null
pubmed: 29986436
Materials (Basel). 2018 Sep 04;11(9):null
pubmed: 30181475
Nanomaterials (Basel). 2018 Oct 09;8(10):null
pubmed: 30304823
Int J Biol Macromol. 2019 Jun 8;136:106-114
pubmed: 31185240
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Biochem J. 1976 Aug 1;157(2):301-6
pubmed: 962868