All tubulins are not alike: Heterodimer dissociation differs among different biological sources.
analytical ultracentrifugation
cytoskeleton
dimerization
dissociation constant
fluorescence
heterologous dimerization
monomer
non-interacting surface (NIS)
protein conformation
tubulin
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
28 06 2019
28 06 2019
Historique:
received:
14
02
2019
revised:
10
05
2019
pubmed:
22
5
2019
medline:
28
2
2020
entrez:
22
5
2019
Statut:
ppublish
Résumé
Tubulin, the subunit of microtubules, is a noncovalent heterodimer composed of one α- and one β-tubulin monomer. Both tubulins are encoded by multiple genes or composed of different isotypes, which are differentially expressed in different tissues and in development. Tubulin αβ dimers are found throughout the eukaryotes and, although very similar, are known to differ among organisms. We seek to investigate tubulins from different tissues and different organisms for a basic physical characteristic: heterodimer stability and monomer exchange between heterodimers. We previously showed that mammalian brain tubulin heterodimers reversibly dissociate, following the mass action law. Dissociation yields native monomers that can exchange with added tubulin to form new heterodimers. Here, we compared the dissociation of tubulins from multiple sources, including mammalian (rat) brain, cultured human cells (HeLa cells), chicken brain, chicken erythrocytes, and the protozoan
Identifiants
pubmed: 31110044
pii: S0021-9258(20)35440-5
doi: 10.1074/jbc.RA119.007973
pmc: PMC6664194
doi:
Substances chimiques
Tubulin
0
Banques de données
PDB
['1JFF', '1SA0']
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
10315-10324Références
Anal Biochem. 1992 Nov 1;206(2):215-25
pubmed: 1443589
Nature. 2005 May 26;435(7041):523-7
pubmed: 15917813
Biophys J. 2008 Mar 1;94(5):1796-806
pubmed: 18024502
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4962-6
pubmed: 368805
Mol Biol Cell. 2001 Jul;12(7):2185-94
pubmed: 11452012
Biochemistry. 2003 Oct 21;42(41):12019-27
pubmed: 14556633
Methods. 2013 Mar;59(3):349-62
pubmed: 23296019
J Cell Biol. 1987 Nov;105(5):2191-201
pubmed: 3680377
J Cell Biol. 1987 Dec;105(6 Pt 2):3065-73
pubmed: 3693406
PLoS One. 2009 Sep 29;4(9):e7253
pubmed: 19787042
J Cell Biol. 1993 Sep;122(6):1301-10
pubmed: 8104191
Elife. 2015 Jul 24;4:
pubmed: 26208336
Nat Rev Mol Cell Biol. 2011 Nov 16;12(12):773-86
pubmed: 22086369
Cytoskeleton (Hoboken). 2016 Oct;73(10):521-550
pubmed: 26934450
J Mol Biol. 2014 Jul 15;426(14):2632-52
pubmed: 24768922
Biochemistry. 1988 Mar 22;27(6):1869-80
pubmed: 2897863
J Biol Chem. 2016 Apr 22;291(17):9281-94
pubmed: 26934918
EMBO J. 2000 Nov 15;19(22):5971-9
pubmed: 11080144
Biophys J. 2000 Mar;78(3):1606-19
pubmed: 10692345
J Cell Biol. 1990 Jan;110(1):97-104
pubmed: 2295686
Methods Cell Biol. 2013;115:155-71
pubmed: 23973072
J Cell Biol. 2016 May 23;213(4):425-33
pubmed: 27185835
Mol Biol Cell. 2002 Jun;13(6):2120-31
pubmed: 12058074
Biochemistry. 1994 Feb 1;33(4):885-93
pubmed: 8305436
J Biol Chem. 2006 May 12;281(19):13628-35
pubmed: 16554299
Anal Biochem. 1995 Jul 1;228(2):343-8
pubmed: 8572317
J Cell Biol. 2005 Apr 25;169(2):233-8
pubmed: 15851515
Methods Cell Biol. 2010;95:17-32
pubmed: 20466127
Biochemistry. 1989 Jul 25;28(15):6518-24
pubmed: 2506927
J Biol Chem. 1998 Jan 2;273(1):167-76
pubmed: 9417061
Biochemistry. 1968 Dec;7(12):4466-79
pubmed: 5700666
Genome Biol Evol. 2014 Aug 27;6(9):2274-88
pubmed: 25169981
PLoS One. 2013 Dec 17;8(12):e83439
pubmed: 24358283
Protein Expr Purif. 1991 Oct-Dec;2(5-6):390-3
pubmed: 1821814
J Cell Biol. 1985 Sep;101(3):852-61
pubmed: 3839797
Anal Chem. 2014 Mar 18;86(6):3181-7
pubmed: 24552356
J Biol Chem. 1988 Nov 5;263(31):16023-31
pubmed: 3182779
Cell. 2014 Nov 20;159(5):1042-1055
pubmed: 25416944
FEBS Lett. 1980 Jan 28;110(1):77-9
pubmed: 7353666
J Mol Biol. 2017 Nov 24;429(23):3696-3716
pubmed: 28970104
Biochemistry. 1991 Apr 9;30(14):3511-7
pubmed: 2012810
J Biol Chem. 2001 Nov 2;276(44):41023-8
pubmed: 11535601
Elife. 2017 Oct 23;6:
pubmed: 29058671
J R Soc Interface. 2012 Dec 12;10(79):20120835
pubmed: 23235262
Nat Protoc. 2017 Sep;12(9):1777-1791
pubmed: 28771239
Int Rev Cell Mol Biol. 2013;302:41-185
pubmed: 23351710
Biochemistry. 1992 Oct 13;31(40):9709-16
pubmed: 1390747
Nat Cell Biol. 2014 Apr;16(4):335-44
pubmed: 24633327
Biophys J. 2012 May 2;102(9):2176-85
pubmed: 22824282
Methods Enzymol. 2015;562:109-33
pubmed: 26412649