Immunohistochemical study of α-smooth muscle actin expression in oral leukoplakia and oral squamous cell carcinoma.
Myofibroblasts
oral leukoplakia
oral squamous cell carcinoma
α-smooth muscle actin
Journal
Journal of oral and maxillofacial pathology : JOMFP
ISSN: 0973-029X
Titre abrégé: J Oral Maxillofac Pathol
Pays: India
ID NLM: 101227995
Informations de publication
Date de publication:
Historique:
entrez:
22
5
2019
pubmed:
22
5
2019
medline:
22
5
2019
Statut:
ppublish
Résumé
It is important to evaluate the role of stromal myofibroblasts (MFs) in carcinogenesis and also as a predictive marker for lymph node (LN) metastasis at the invasive front of oral squamous cell carcinoma (OSCC). To demonstrate the expression of α-smooth muscle actin (α-SMA) by MFs in the tissues of oral leukoplakia (OL) with dysplasia and OSCC. To record and compare the distribution of MFs in OSCC with LN metastasis and without LN metastasis. Fifty paraffin-embedded tissue blocks with 10 cases of normal oral mucosa, 10 cases of OL with dysplasia and 30 diagnosed cases of OSCCs were studied. The samples were subjected to heat-induced antigen retrieval method followed by staining using primary mouse monoclonal antibodies against α-SMA and visualized using super sensitive polymer-HRP detection system. Descriptive statistical analysis and ANOVA test were used for statistical analysis. There was no α-SMA expression in normal oral mucosa or in OL with dysplasia. All tissues of OSCC were positive for α-SMA expression. The difference in the expression between OL with dysplasia and OSCC was statistically significant ( α-SMA expression by MFs in OSCCs indicates its role in tumor growth and invasion. The mean α-SMA count was found to correlate with tumor invasiveness and locoregional LN metastasis.
Identifiants
pubmed: 31110418
doi: 10.4103/jomfp.JOMFP_94_18
pii: JOMFP-23-59
pmc: PMC6503784
doi:
Types de publication
Journal Article
Langues
eng
Pagination
59-64Déclaration de conflit d'intérêts
There are no conflicts of interest.
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