A novel fungal GH30 xylanase with xylobiohydrolase auxiliary activity.
GH30 xylanase
Glucuronoxylan
Thermothelomyces thermophila
Xylobiohydrolase
Xylooligosaccharides
Journal
Biotechnology for biofuels
ISSN: 1754-6834
Titre abrégé: Biotechnol Biofuels
Pays: England
ID NLM: 101316935
Informations de publication
Date de publication:
2019
2019
Historique:
received:
05
02
2019
accepted:
29
04
2019
entrez:
22
5
2019
pubmed:
22
5
2019
medline:
22
5
2019
Statut:
epublish
Résumé
The main representatives of hemicellulose are xylans, usually decorated β-1,4-linked d-xylose polymers, which are hydrolyzed by xylanases. The efficient utilization and complete hydrolysis of xylans necessitate the understanding of the mode of action of xylan degrading enzymes. The glycoside hydrolase family 30 (GH30) xylanases comprise a less studied group of such enzymes, and differences regarding the substrate recognition have been reported between fungal and bacterial GH30 xylanases. Besides their role in the utilization of lignocellulosic biomass for bioenergy, such enzymes could be used for the tailored production of prebiotic xylooligosaccharides (XOS) due to their substrate specificity. The expression of a putative GH30_7 xylanase from the fungus Hereby, we reported the heterologous production and biochemical characterization of a novel fungal GH30 xylanase exhibiting endo- and exo-xylanase activity. To date, considering its novel catalytic properties,
Sections du résumé
BACKGROUND
BACKGROUND
The main representatives of hemicellulose are xylans, usually decorated β-1,4-linked d-xylose polymers, which are hydrolyzed by xylanases. The efficient utilization and complete hydrolysis of xylans necessitate the understanding of the mode of action of xylan degrading enzymes. The glycoside hydrolase family 30 (GH30) xylanases comprise a less studied group of such enzymes, and differences regarding the substrate recognition have been reported between fungal and bacterial GH30 xylanases. Besides their role in the utilization of lignocellulosic biomass for bioenergy, such enzymes could be used for the tailored production of prebiotic xylooligosaccharides (XOS) due to their substrate specificity.
RESULTS
RESULTS
The expression of a putative GH30_7 xylanase from the fungus
CONCLUSIONS
CONCLUSIONS
Hereby, we reported the heterologous production and biochemical characterization of a novel fungal GH30 xylanase exhibiting endo- and exo-xylanase activity. To date, considering its novel catalytic properties,
Identifiants
pubmed: 31110561
doi: 10.1186/s13068-019-1455-2
pii: 1455
pmc: PMC6511221
doi:
Types de publication
Journal Article
Langues
eng
Pagination
120Déclaration de conflit d'intérêts
Not applicable.All authors consented on the publication of this work.The authors declare that they have no competing interests.
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