Functional expression and activity screening of all human cytochrome P450 enzymes in fission yeast.
Homo sapiens
cytochrome P450
fission yeast
functional expression
gene library
pharmacology
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
07
04
2019
revised:
29
04
2019
accepted:
10
05
2019
pubmed:
22
5
2019
medline:
27
6
2020
entrez:
22
5
2019
Statut:
ppublish
Résumé
Here, a complete set of recombinant fission yeast strains that coexpress each of the 57 human cytochrome P450 (CYP) enzymes together with their natural human electron transfer partner(s) was cloned. This strain collection was tested with two luminogenic probe substrates, and 31 human CYPs (including the orphan enzymes CYP2A7, CYP4A22 and CYP20A1) were found to metabolize at least one of these. Since other substrates are known for the remaining enzymes, all human CYPs are now shown to be active. Interestingly, CYP5A1 was found for the first time to work on a substrate other than prostaglandin H
Identifiants
pubmed: 31111477
doi: 10.1002/1873-3468.13441
doi:
Substances chimiques
Cytochrome P-450 Enzyme System
9035-51-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1372-1380Informations de copyright
© 2019 Federation of European Biochemical Societies.