A micellar electrokinetic chromatography approach using diastereomeric derivatization and a volatile surfactant for the enantioselective separation of selenomethionine.
(+)-1-(9-fluorenyl)ethyl chloroformate
Ammonium perfluorooctanoate
Chiral separation
Food supplements
MEKC
Selenomethionine
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
02
02
2019
revised:
16
05
2019
accepted:
18
05
2019
pubmed:
22
5
2019
medline:
12
2
2020
entrez:
22
5
2019
Statut:
ppublish
Résumé
A MEKC methodology with UV detection was developed for the enantioselective separation of selenomethionine (SeMet). The use of (+)-1-(9-fluorenyl)ethyl chloroformate (FLEC) as chiral derivatization reagent to form SeMet diastereomers enabled their subsequent separation using ammonium perfluorooctanoate (APFO) as a volatile pseudostationary phase. The effect of APFO concentration and pH, temperature, injection volume, and derivatization conditions (time and FLEC/SeMet ratio) were evaluated in order to select the best separation conditions. A chiral resolution of 4.4 for DL-SeMet was achieved in less than 6 min using 100 mM APFO at pH 8.5 as electrophoretic buffer. Satisfactory results were obtained in terms of linearity, precision (RSD from 3.4 to 5.1% for migration times and from 1.8 to 4.6% for corrected peak areas), accuracy, and LODs (3.1 × 10
Identifiants
pubmed: 31111508
doi: 10.1002/elps.201900077
doi:
Substances chimiques
Caprylates
0
Fluorenes
0
Fluorocarbons
0
Surface-Active Agents
0
perfluorooctanoic acid
947VD76D3L
Selenomethionine
964MRK2PEL
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1951-1958Subventions
Organisme : Ministry of Economy and Competitiveness
ID : CTQ2016-76368-P
Pays : International
Organisme : Comunidad of Madrid (Spain) and European funding
ID : S2018/BAA-4393
Pays : International
Organisme : Comunidad of Madrid (Spain) and European funding
ID : AVANSECAL-II-CM
Pays : International
Organisme : Spanish Ministry of Economy and Competitiveness
ID : CTQ2016-76368-P
Pays : International
Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.