Plasma amino acid profiles in dogs with inflammatory bowel disease.
canine
chronic enteropathy
gastrointestinal disease
metabolism
Journal
Journal of veterinary internal medicine
ISSN: 1939-1676
Titre abrégé: J Vet Intern Med
Pays: United States
ID NLM: 8708660
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
22
11
2018
accepted:
08
05
2019
pubmed:
22
5
2019
medline:
4
12
2019
entrez:
22
5
2019
Statut:
ppublish
Résumé
Lymphocytic-plasmacytic enteritis is the common form of idiopathic inflammatory bowel disease (IBD) in dogs. In human IBD, disturbances of amino acid metabolism have been demonstrated to be involved in the pathophysiology of IBD. Therefore, plasma amino acid profile might represent a novel marker of human IBD. To determine the plasma amino acid profiles of dogs with IBD and its usefulness as a novel marker of IBD in dogs. Fasting blood plasma was obtained from 10 dogs with IBD and 12 healthy dogs. All IBD dogs were prospectively included in this study, and heparinized blood samples were collected. The plasma concentrations of 21 amino acids were determined using the ninhydrin method. The relationships among the plasma amino acid concentrations and plasma C-reactive protein (CRP) concentration, canine chronic enteropathy clinical activity index (CCECAI), and overall World Small Animal Veterinary Association (WSAVA) score were investigated. Median concentration (nmol/mL) of methionine [46.2; range, 30.0-59.3], proline [119.4; range, 76.7-189.2], serine [115.1; range, 61.4-155.9], and tryptophan [17.4; range, 11.9-56.3]) were significantly lower than in control dogs [62.6; range, 51.0-83.6, 199.1; range, 132.5-376.7, 164.3; range, 124.7-222.9, and 68.3; range, 35.7-94.8, respectively]. A negative correlation was identified between the plasma serine concentration and CCECAI (r Plasma serine concentration might represent a novel maker of IBD in dogs.
Sections du résumé
BACKGROUND
BACKGROUND
Lymphocytic-plasmacytic enteritis is the common form of idiopathic inflammatory bowel disease (IBD) in dogs. In human IBD, disturbances of amino acid metabolism have been demonstrated to be involved in the pathophysiology of IBD. Therefore, plasma amino acid profile might represent a novel marker of human IBD.
OBJECTIVES
OBJECTIVE
To determine the plasma amino acid profiles of dogs with IBD and its usefulness as a novel marker of IBD in dogs.
ANIMALS
METHODS
Fasting blood plasma was obtained from 10 dogs with IBD and 12 healthy dogs.
METHODS
METHODS
All IBD dogs were prospectively included in this study, and heparinized blood samples were collected. The plasma concentrations of 21 amino acids were determined using the ninhydrin method. The relationships among the plasma amino acid concentrations and plasma C-reactive protein (CRP) concentration, canine chronic enteropathy clinical activity index (CCECAI), and overall World Small Animal Veterinary Association (WSAVA) score were investigated.
RESULTS
RESULTS
Median concentration (nmol/mL) of methionine [46.2; range, 30.0-59.3], proline [119.4; range, 76.7-189.2], serine [115.1; range, 61.4-155.9], and tryptophan [17.4; range, 11.9-56.3]) were significantly lower than in control dogs [62.6; range, 51.0-83.6, 199.1; range, 132.5-376.7, 164.3; range, 124.7-222.9, and 68.3; range, 35.7-94.8, respectively]. A negative correlation was identified between the plasma serine concentration and CCECAI (r
CONCLUSIONS AND CLINICAL IMPORTANCE
CONCLUSIONS
Plasma serine concentration might represent a novel maker of IBD in dogs.
Identifiants
pubmed: 31111561
doi: 10.1111/jvim.15525
pmc: PMC6639477
doi:
Substances chimiques
Amino Acids
0
Biomarkers
0
Serine
452VLY9402
C-Reactive Protein
9007-41-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1602-1607Informations de copyright
© 2019 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
Références
Turk J Gastroenterol. 2013;24(1):30-5
pubmed: 23794341
J Comp Pathol. 2008 Feb-Apr;138 Suppl 1:S1-43
pubmed: 18336828
PLoS One. 2012;7(1):e31131
pubmed: 22303484
J Vet Intern Med. 2009 May-Jun;23(3):559-63
pubmed: 19645841
Br J Nutr. 2018 Dec;120(12):1321-1331
pubmed: 30375295
Proc Natl Acad Sci U S A. 2005 Jun 7;102(23):8363-8
pubmed: 15919826
J Vet Intern Med. 2018 May;32(3):1026-1032
pubmed: 29604114
Amino Acids. 2015 Oct;47(10):2127-41
pubmed: 25501277
J Vet Intern Med. 2003 Jan-Feb;17(1):8-20
pubmed: 12564722
Gut Microbes. 2015;6(1):33-47
pubmed: 25531678
PLoS One. 2015 Oct 16;10(10):e0140716
pubmed: 26474176
Biomed Res Int. 2018 Feb 26;2018:9171905
pubmed: 29682569
J Agric Food Chem. 2003 Jun 4;51(12):3668-74
pubmed: 12769543
J Transl Med. 2015 Jan 27;13:35
pubmed: 25622826
J Vet Intern Med. 2007 Jul-Aug;21(4):700-8
pubmed: 17708389
Vet Dermatol. 2002 Aug;13(4):177-86
pubmed: 12174180
J Nutr Biochem. 2010 Jun;21(6):468-75
pubmed: 19428234
PLoS One. 2013 Jun 20;8(6):e66074
pubmed: 23840402
Vet Immunol Immunopathol. 2012 Mar 15;146(1):87-91
pubmed: 22333286
J Vet Intern Med. 2019 Jul;33(4):1602-1607
pubmed: 31111561
J Vet Med Sci. 2014 Aug;76(8):1119-27
pubmed: 24829081
Nature. 2007 Jul 26;448(7152):427-34
pubmed: 17653185
Infect Immun. 2007 Jan;75(1):164-74
pubmed: 17043106
J Vet Sci. 2012 Dec;13(4):433-6
pubmed: 23271187
J Vet Intern Med. 2010 Jan-Feb;24(1):10-26
pubmed: 20391635
Semin Immunopathol. 2012 Jan;34(1):133-49
pubmed: 21971685
Vet Immunol Immunopathol. 2012 Aug 15;148(3-4):226-35
pubmed: 22648046
Circ J. 2010 Nov;74(11):2434-40
pubmed: 20834187
J Anim Sci. 2004 Apr;82(4):1091-9
pubmed: 15080331
Nutrients. 2017 Aug 23;9(9):
pubmed: 28832517