Effect of Turkish Propolis on miRNA Expression, Cell Cycle, and Apoptosis in Human Breast Cancer (MCF-7) Cells.
Anti-Infective Agents
/ pharmacology
Apoptosis
Biological Products
/ pharmacology
Breast Neoplasms
/ drug therapy
Cell Cycle Checkpoints
Cell Line, Tumor
Cell Proliferation
Female
Gene Expression Regulation, Neoplastic
/ drug effects
Humans
MCF-7 Cells
Membrane Potential, Mitochondrial
/ drug effects
MicroRNAs
/ genetics
Propolis
/ pharmacology
Tumor Suppressor Protein p53
/ genetics
Turkey
Journal
Nutrition and cancer
ISSN: 1532-7914
Titre abrégé: Nutr Cancer
Pays: United States
ID NLM: 7905040
Informations de publication
Date de publication:
2020
2020
Historique:
pubmed:
22
5
2019
medline:
12
9
2020
entrez:
22
5
2019
Statut:
ppublish
Résumé
Enriched in flavonoid compounds, phenol acids, and terpene derivatives, propolis has been shown to regulate apoptosis signaling pathways and alter the expression of microRNAs (miRNAs). In the present study, it has been aimed to examine the effects of Turkish propolis on miRNA levels of breast cancer (MCF-7) cells, and its relationship with cell proliferation and apoptosis. Cytotoxic activity of ethanolic propolis extract (EEP) was evaluated using MTT assay. Mechanisms involved in the cytotoxic action of Turkish propolis in MCF-7 cells were investigated with regard to apoptosis and cell cycle using flow cytometry and western blot. Mitochondrial membrane potential (MMP) were evaluated by spectrofluorometric method. miRNA levels were detected by qRT-PCR method. EEP exhibited selective toxicity against MCF-7 cells compared to normal fibroblast cells. EEP increased the cell cycle arrest at the G
Identifiants
pubmed: 31112051
doi: 10.1080/01635581.2019.1616100
doi:
Substances chimiques
Anti-Infective Agents
0
Biological Products
0
MicroRNAs
0
TP53 protein, human
0
Tumor Suppressor Protein p53
0
Propolis
9009-62-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM