Antagonistic activity of recombinant Lactococcus lactis NZ1330 on the adhesion properties of Escherichia coli causing urinary tract infection.
Antibiosis
Bacterial Adhesion
/ genetics
Bile Acids and Salts
Caco-2 Cells
Cell Aggregation
Escherichia coli
/ growth & development
Gene Expression Regulation, Bacterial
Genes, Bacterial
/ genetics
Host Microbial Interactions
Humans
Hydrogen-Ion Concentration
Hydrophobic and Hydrophilic Interactions
Intestines
/ microbiology
Lactococcus lactis
/ genetics
Probiotics
/ pharmacology
Transformation, Bacterial
Urinary Tract Infections
/ microbiology
Adhesion to Caco-2
Antagonistic activity
Infection disease
Lactococcus lactis
Journal
Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
18
04
2019
revised:
16
05
2019
accepted:
17
05
2019
pubmed:
22
5
2019
medline:
14
8
2019
entrez:
22
5
2019
Statut:
ppublish
Résumé
Death from infectious diseases has caused concerns about increases in the resistance of pathogens, impelling researchers to create novel therapeutic solutions. The management of intestinal tract problems has been the advance use of probiotics in medicine. The aim of this study was evaluate the physicochemical cell surface and adhesion properties of recombinant Lacotococcus lactis NZ1330 containing Ama r 2 gene, followed by the assessment of the antagonistic activity of this strain against the Escherichia coli causing urinary tract infection (UTI) in humans. For this purpose, cloning and expression of Ama r 2 gene were done. Afterwards, acid and bile resistance, which are the primary characteristics of any probiotic, were evaluated. The r-L. lactis NZ1330 was examined for the physicochemical properties of cell surfaces and the adhesion properties against Escherichia coli. Furthermore, the potential of the recombinant strain to adhere to adenocarcinoma intestinal cell line, Caco-2 cells, as well as the antagonistic properties of r-L. lactis NZ1330 against E. coli was investigated. r-L. lactis NZ1330 was capable of surviving at low pH and different concentrations of bile salts. 40.1% hydrophobicity, 36.5% auto-aggregation and 14.4% co-aggregation were observed for this strain. The adhesion level of r-L. lactis NZ1330 was 5.7% which was also confirmed by scanning electron microscopy (SEM). r-L. lactis NZ1330 was able to compete, inhibit and displace the adhesion of Escherichia coli to Caco-2 cells. r-L. lactis NZ1330 was considered to be a reliable probiotic alternative by showing these desirable properties. Results revealed that Ama r 2 gene expression had no effect on the positive probiotic properties of L. lactis NZ1330, proving this strain could be a suitable probiotic host for the expression of this allergen.
Identifiants
pubmed: 31112774
pii: S0882-4010(19)30709-0
doi: 10.1016/j.micpath.2019.103547
pii:
doi:
Substances chimiques
Bile Acids and Salts
0
Types de publication
Journal Article
Langues
eng
Pagination
103547Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.