Molecular epidemiology of coagulase-negative
Coagulase negative Staphylococcus (CNS)
Epidemiology
Genetic relatedness
Mastitis pathogens
Phylogeny
Pulse-field gel electrophoresis (PFGE)
Journal
PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425
Informations de publication
Date de publication:
2019
2019
Historique:
received:
08
10
2018
accepted:
08
03
2019
entrez:
24
5
2019
pubmed:
24
5
2019
medline:
24
5
2019
Statut:
epublish
Résumé
Coagulase negative A total of 604 CNS isolates were cultured from milk samples collected during a dry-cow treatment clinical trial conducted on 6 dairy herds in 4 states in the US. All the study cows were randomized to receive 1 of the 3 different intra-mammary antimicrobial infusions (Quatermaster, Spectramast DC or ToMorrow Dry Cow) at dry-off. Milk samples were collected at dry-off, calving (0-6 days in milk, DIM), post-calving (7-13 DIM) and at mastitis events within the first 100 DIM. The CNS isolates were identified to species level by partial sequencing of the The major CNS species identified were The observed association between genetic and epidemiological distributions indicated animal-adapted nature of four CNS species, suggesting possible host-adapted and environmental transmission of these species. Multi-stage isolation of the same udder quarter strain was evidence for chronic intra-mammary infection. The different CNS species and strains circulating on US dairy herds were genetically diverse. Four species identified were likely udder-adapted pathogens, 2 of which caused persistent infection. Our findings are important in guiding the design of effective mastitis control strategies.
Sections du résumé
BACKGROUND
BACKGROUND
Coagulase negative
METHODOLOGY
METHODS
A total of 604 CNS isolates were cultured from milk samples collected during a dry-cow treatment clinical trial conducted on 6 dairy herds in 4 states in the US. All the study cows were randomized to receive 1 of the 3 different intra-mammary antimicrobial infusions (Quatermaster, Spectramast DC or ToMorrow Dry Cow) at dry-off. Milk samples were collected at dry-off, calving (0-6 days in milk, DIM), post-calving (7-13 DIM) and at mastitis events within the first 100 DIM. The CNS isolates were identified to species level by partial sequencing of the
RESULTS
RESULTS
The major CNS species identified were
DISCUSSION
CONCLUSIONS
The observed association between genetic and epidemiological distributions indicated animal-adapted nature of four CNS species, suggesting possible host-adapted and environmental transmission of these species. Multi-stage isolation of the same udder quarter strain was evidence for chronic intra-mammary infection.
CONCLUSION
CONCLUSIONS
The different CNS species and strains circulating on US dairy herds were genetically diverse. Four species identified were likely udder-adapted pathogens, 2 of which caused persistent infection. Our findings are important in guiding the design of effective mastitis control strategies.
Identifiants
pubmed: 31119068
doi: 10.7717/peerj.6749
pii: 6749
pmc: PMC6507897
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e6749Déclaration de conflit d'intérêts
The authors declare there are no competing interests.
Références
J Clin Microbiol. 2002 Apr;40(4):1333-8
pubmed: 11923353
Vet Clin North Am Food Anim Pract. 2003 Mar;19(1):171-85, vii
pubmed: 12682941
J Vet Pharmacol Ther. 2003 Jun;26(3):193-8
pubmed: 12755903
J Clin Microbiol. 1992 Aug;30(8):2169-72
pubmed: 1500528
J Clin Microbiol. 2005 Mar;43(3):1045-50
pubmed: 15750058
Vet Microbiol. 2006 Jun 15;115(1-3):199-207
pubmed: 16527434
J Dairy Sci. 2006 Jul;89(7):2542-51
pubmed: 16772573
J Dairy Res. 2008 Nov;75(4):422-9
pubmed: 18700996
Vet Microbiol. 2009 Feb 16;134(1-2):9-14
pubmed: 18842362
Vet Microbiol. 2009 Feb 16;134(1-2):3-8
pubmed: 18848410
Vet Microbiol. 2009 Feb 16;134(1-2):20-8
pubmed: 18930607
Vet Microbiol. 2009 Feb 16;134(1-2):95-9
pubmed: 18938048
Vet Microbiol. 2009 Feb 16;134(1-2):65-72
pubmed: 18950962
Vet Microbiol. 2009 Feb 16;134(1-2):73-81
pubmed: 18950969
Vet Microbiol. 2009 Feb 16;134(1-2):37-44
pubmed: 18977613
J Dairy Sci. 2009 Oct;92(10):4962-70
pubmed: 19762813
Clin Microbiol Infect. 2011 Jan;17(1):44-9
pubmed: 20132252
J Dairy Sci. 2011 Jan;94(1):250-61
pubmed: 21183035
J Dairy Sci. 2011 May;94(5):2329-40
pubmed: 21524522
J Dairy Sci. 2011 Jun;94(6):2933-44
pubmed: 21605763
J Dairy Res. 2012 May;79(2):129-34
pubmed: 22067091
Vet Microbiol. 2012 Sep 14;159(1-2):171-80
pubmed: 22503603
Vet Clin North Am Food Anim Pract. 2012 Jul;28(2):149-63
pubmed: 22664200
Can J Vet Res. 2012 Oct;76(4):268-74
pubmed: 23543952
J Dairy Sci. 2013 Jul;96(7):4419-35
pubmed: 23628244
J Dairy Sci. 2014;97(1):270-84
pubmed: 24183691
J Dairy Res. 2014 May;81(2):208-14
pubmed: 24594229
Ir Vet J. 2014 Jun 02;67(1):12
pubmed: 24917926
Vet J. 2015 Jan;203(1):44-51
pubmed: 25467994
J Dairy Sci. 2015 May;98(5):3071-8
pubmed: 25726098
J Dairy Sci. 2015 Aug;98(8):5448-53
pubmed: 26051318
Rev Argent Microbiol. 2016 Jan-Mar;48(1):50-6
pubmed: 26935912
J Dairy Sci. 2016 Aug;99(8):6457-6469
pubmed: 27236763
J Dairy Sci. 1989 Jul;72(7):1886-92
pubmed: 2778172
J Dairy Sci. 2017 Jan;100(1):629-642
pubmed: 27865514
J Dairy Sci. 2017 Jul;100(7):5613-5627
pubmed: 28456402
Acta Vet Scand. 2017 May 25;59(1):33
pubmed: 28545485
J Dairy Sci. 2018 Jun;101(6):5388-5403
pubmed: 29525303
J Clin Microbiol. 1995 Sep;33(9):2233-9
pubmed: 7494007