Recombinase polymerase amplification assay for rapid detection of Monkeypox virus.
Monkeypox Virus
Recombinase polymerase amplification assay
mobile suitcase
point of need
rapid detection system
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
11
12
2018
revised:
04
03
2019
accepted:
28
03
2019
pubmed:
28
5
2019
medline:
18
12
2019
entrez:
26
5
2019
Statut:
ppublish
Résumé
In this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) using recombinase polymerase amplification (RPA) assay targeting the G2R gene was developed. MPXV, an Orthopoxvirus, is a zoonotic dsDNA virus, which is listed as a biothreat agent. RPA was operated at a single constant temperature of 42°C and produced results within 3 to 10 minutes. The MPXV-RPA-assay was highly sensitive with a limit of detection of 16 DNA molecules/μl. The clinical performance of the MPXV-RPA-assay was tested using 47 sera and whole blood samples from humans collected during the recent MPXV outbreak in Nigeria as well as 48 plasma samples from monkeys some of which were experimentally infected with MPXV. The specificity of the MPXV-RPA-assay was 100% (50/50), while the sensitivity was 95% (43/45). This new MPXV-RPA-assay is fast and can be easily utilised at low resource settings using a solar powered mobile suitcase laboratory.
Identifiants
pubmed: 31126795
pii: S0732-8893(18)30746-6
doi: 10.1016/j.diagmicrobio.2019.03.015
pmc: PMC9629024
pii:
doi:
Substances chimiques
DNA, Viral
0
Recombinases
0
Viral Proteins
0
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
41-45Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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