Sphingosine kinase 2 is a negative regulator of inflammatory macrophage activation.
Autophagy
Inflammation
Macrophage
Sphingolipids
Journal
Biochimica et biophysica acta. Molecular and cell biology of lipids
ISSN: 1879-2618
Titre abrégé: Biochim Biophys Acta Mol Cell Biol Lipids
Pays: Netherlands
ID NLM: 101731727
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
20
09
2018
revised:
15
05
2019
accepted:
20
05
2019
pubmed:
28
5
2019
medline:
3
3
2020
entrez:
26
5
2019
Statut:
ppublish
Résumé
Sphingosine kinases (SPHK) generate the sphingolipid sphingosine-1-phosphate, which, among other functions, is a potent regulator of inflammation. While SPHK1 produces S1P to promote inflammatory signaling, the role of SPHK2 is unclear due to divergent findings in studies utilizing gene depletion versus inhibition of catalytic activity. We sought to clarify how SPHK2 affects inflammatory signaling in human macrophages, which are main regulators of inflammation. SPHK2 expression and activity were rapidly decreased within 6 h upon stimulating primary human macrophages with lipopolysaccharide (LPS), but was upregulated after 24 h. At 24 h following LPS stimulation, targeting SPHK2 with the inhibitor ABC294640, a specific siRNA or by using Sphk2
Identifiants
pubmed: 31128248
pii: S1388-1981(18)30300-7
doi: 10.1016/j.bbalip.2019.05.008
pii:
doi:
Substances chimiques
Cytokines
0
Phosphotransferases (Alcohol Group Acceptor)
EC 2.7.1.-
sphingosine kinase 2, human
EC 2.7.1.91
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1235-1246Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.