Oral fluid: Non-invasive alternative for parvovirus B19 diagnosis?
Antibodies, Viral
/ blood
Capsid Proteins
/ genetics
Child
Erythema Infectiosum
/ diagnosis
Female
Humans
Immunoglobulin M
/ blood
Male
Molecular Diagnostic Techniques
Parvovirus B19, Human
/ genetics
Real-Time Polymerase Chain Reaction
Saliva
/ virology
Sample Size
Sensitivity and Specificity
Viral Nonstructural Proteins
/ genetics
Children
Exanthema
Fifth disease
PCR
Parvovirus B19
Serology
Journal
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
ISSN: 1873-5967
Titre abrégé: J Clin Virol
Pays: Netherlands
ID NLM: 9815671
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
03
09
2018
revised:
18
04
2019
accepted:
17
05
2019
pubmed:
28
5
2019
medline:
17
6
2020
entrez:
26
5
2019
Statut:
ppublish
Résumé
Infections with parvovirus B19 (B19V) have been associated with a wide range of disease manifestations of which erythema infectiosum (fifth disease) in children is most common. Clinical signs following infection of children with B19V can be similar to measles and rubella. Laboratory detection of B19V infections is based on detection of B19V-specific IgM antibodies by enzyme immunoassay (IgM-EIA) and/or B19V DNA by quantitative PCR (qPCR) on blood samples. The need for invasive sampling can be a barrier for public health diagnostics. To evaluate the use of a dual target B19V-qPCR directed against the NS1 and VP2 of B19V on oral fluid samples as a non-invasive alternative for laboratory diagnosis of B19V infections in children below 12 years of age with exanthema. Oral fluid and serum samples were collected from 116 children with exanthema. All serum samples were tested by IgM-EIA/IgG-EIA, while all oral fluid and 56 serum samples were tested by B19V-qPCR. B19V-specific IgM antibodies were detected in 25 of 116 children in the study. B19V DNA was detected in oral fluid in 17 of the 25 children who were IgM positive, as well as two children who were IgM-equivocal or negative. The child with the equivocal IgM had a high quantity of B19V DNA in oral fluid (7 log IU/ml), compatible with an acute B19V infection. The IgM-negative child was IgG-positive and 4 log IU/ml B19V DNA was detected in the oral fluid sample, suggesting an acute infection and a falsely negative IgM. Sample size calculations indicated that oral fluid samples for qPCR should be collected from 2 to 3 children during outbreaks of exanthema to achieve similar sensitivity as IgM-EIA for one child (≥0.9) to confirm or exclude B19V. Results indicate that oral fluid samples are a suitable public health alternative for detection of B19V infections, potentially lowering the barriers for sampling.
Sections du résumé
BACKGROUND
Infections with parvovirus B19 (B19V) have been associated with a wide range of disease manifestations of which erythema infectiosum (fifth disease) in children is most common. Clinical signs following infection of children with B19V can be similar to measles and rubella. Laboratory detection of B19V infections is based on detection of B19V-specific IgM antibodies by enzyme immunoassay (IgM-EIA) and/or B19V DNA by quantitative PCR (qPCR) on blood samples. The need for invasive sampling can be a barrier for public health diagnostics.
OBJECTIVES
To evaluate the use of a dual target B19V-qPCR directed against the NS1 and VP2 of B19V on oral fluid samples as a non-invasive alternative for laboratory diagnosis of B19V infections in children below 12 years of age with exanthema.
STUDY DESIGN
Oral fluid and serum samples were collected from 116 children with exanthema. All serum samples were tested by IgM-EIA/IgG-EIA, while all oral fluid and 56 serum samples were tested by B19V-qPCR.
RESULTS
B19V-specific IgM antibodies were detected in 25 of 116 children in the study. B19V DNA was detected in oral fluid in 17 of the 25 children who were IgM positive, as well as two children who were IgM-equivocal or negative. The child with the equivocal IgM had a high quantity of B19V DNA in oral fluid (7 log IU/ml), compatible with an acute B19V infection. The IgM-negative child was IgG-positive and 4 log IU/ml B19V DNA was detected in the oral fluid sample, suggesting an acute infection and a falsely negative IgM. Sample size calculations indicated that oral fluid samples for qPCR should be collected from 2 to 3 children during outbreaks of exanthema to achieve similar sensitivity as IgM-EIA for one child (≥0.9) to confirm or exclude B19V.
CONCLUSIONS
Results indicate that oral fluid samples are a suitable public health alternative for detection of B19V infections, potentially lowering the barriers for sampling.
Identifiants
pubmed: 31128380
pii: S1386-6532(19)30117-9
doi: 10.1016/j.jcv.2019.05.008
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Capsid Proteins
0
Immunoglobulin M
0
NS1 protein, parvovirus
0
Viral Nonstructural Proteins
0
capsid protein VP2, parvovirus B19
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5-10Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.