Road Map for Development of Stem Cell-Based Alternative Test Methods.
alternative testing strategies
cardiotoxicity
developmental toxicity
drug discovery
hepatotoxicity
nephrotoxicity
neurotoxicity
pluripotent stem cells
Journal
Trends in molecular medicine
ISSN: 1471-499X
Titre abrégé: Trends Mol Med
Pays: England
ID NLM: 100966035
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
09
01
2019
revised:
28
03
2019
accepted:
02
04
2019
pubmed:
28
5
2019
medline:
9
4
2020
entrez:
28
5
2019
Statut:
ppublish
Résumé
Much progress has been made in establishing strategies for differentiation of induced human pluripotent stem cells (hiPSCs). However, differentiated hiPSCs are not yet routinely used for prediction of toxicity. Here, limiting factors are summarised and possibilities for improvement are discussed, with a focus on hepatocytes, cardiomyocytes, tubular epithelial cells, and developmental toxicity. Moreover, we make recommendations for further fine-tuning of differentiation protocols for hiPSCs to hepatocyte-like cells by comparing individual steps of currently available protocols to the mechanisms occurring during embryonic development. A road map is proposed to facilitate test system development, including a description of the most useful performance metrics.
Identifiants
pubmed: 31130451
pii: S1471-4914(19)30074-7
doi: 10.1016/j.molmed.2019.04.003
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
470-481Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.