Colorectal tumor-on-a-chip system: A 3D tool for precision onco-nanomedicine.
Biomimetics
Caspase 3
/ metabolism
Cell Line, Tumor
Cell Survival
Coculture Techniques
Colorectal Neoplasms
/ diagnosis
Dendrimers
/ chemistry
Gene Expression Regulation, Neoplastic
HCT116 Cells
Humans
Imaging, Three-Dimensional
Ki-67 Antigen
/ metabolism
Lab-On-A-Chip Devices
Matrix Metalloproteinase 1
/ metabolism
Microfluidics
Nanomedicine
/ methods
Nanoparticles
/ chemistry
Precision Medicine
/ methods
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
08
01
2019
accepted:
16
04
2019
entrez:
28
5
2019
pubmed:
28
5
2019
medline:
22
7
2020
Statut:
epublish
Résumé
Awareness that traditional two-dimensional (2D) in vitro and nonrepresentative animal models may not completely emulate the 3D hierarchical complexity of tissues and organs is on the rise. Therefore, posterior translation into successful clinical application is compromised. To address this dearth, on-chip biomimetic microenvironments powered by microfluidic technologies are being developed to better capture the complexity of in vivo pathophysiology. Here, we describe a "tumor-on-a-chip" model for assessment of precision nanomedicine delivery on which we validate the efficacy of drug-loaded nanoparticles in a gradient fashion. The model validation was performed by viability studies integrated with live imaging to confirm the dose-response effect of cells exposed to the CMCht/PAMAM nanoparticle gradient. This platform also enables the analysis at the gene expression level, where a down-regulation of all the studied genes (
Identifiants
pubmed: 31131324
doi: 10.1126/sciadv.aaw1317
pii: aaw1317
pmc: PMC6531003
doi:
Substances chimiques
Dendrimers
0
Ki-67 Antigen
0
PAMAM Starburst
0
CASP3 protein, human
EC 3.4.22.-
Caspase 3
EC 3.4.22.-
MMP1 protein, human
EC 3.4.24.7
Matrix Metalloproteinase 1
EC 3.4.24.7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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