PDGF Modulates BMP2-Induced Osteogenesis in Periosteal Progenitor Cells.
BONE MORPHOGENETIC PROTEIN 2
FRACTURE HEALING
PERIOSTEUM
PLATELET‐DERIVED GROWTH FACTOR
STEM CELLS
Journal
JBMR plus
ISSN: 2473-4039
Titre abrégé: JBMR Plus
Pays: England
ID NLM: 101707013
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
03
08
2018
revised:
23
09
2018
accepted:
03
10
2018
entrez:
28
5
2019
pubmed:
28
5
2019
medline:
28
5
2019
Statut:
epublish
Résumé
BMPs are used in various clinical applications to promote bone formation. The limited success of the BMPs in clinical settings and supraphysiological doses required for their effects prompted us to evaluate the influence of other signaling molecules, specifically platelet-derived growth factor (PDGF) on BMP2-induced osteogenesis. Periosteal cells make a major contribution to fracture healing. We detected broad expression of PDGF receptor beta (PDGFRβ) within the intact periosteum and healing callus during fracture repair. In vitro, periosteum-derived progenitor cells were highly responsive to PDGF as demonstrated by increased proliferation and decreased apoptosis. However, PDGF blocked BMP2-induced osteogenesis by inhibiting the canonical BMP2/Smad pathway and downstream target gene expression. This effect is mediated via PDGFRβ and involves ERK1/2 MAPK and PI3K/AKT signaling pathways. Therapeutic targeting of the PDGFRβ pathway in periosteum-mediated bone repair might have profound implications in the treatment of bone disease in the future.
Identifiants
pubmed: 31131345
doi: 10.1002/jbm4.10127
pii: JBM410127
pmc: PMC6524680
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e10127Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR055607
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR070813
Pays : United States
Informations de copyright
© 2018 The Authors is published by Wiley Periodicals, Inc. on behalf of the American Society for Bone and Mineral Research.
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