The rBC2LCN-positive subpopulation of PC-3 cells exhibits cancer stem-like properties.
Biomarkers, Tumor
/ genetics
Cell Line
Cell Line, Tumor
Gene Expression Profiling
/ methods
Gene Expression Regulation, Neoplastic
Human Embryonic Stem Cells
/ metabolism
Humans
Induced Pluripotent Stem Cells
/ metabolism
Lectins
/ genetics
MCF-7 Cells
Male
Molecular Probes
/ genetics
Neoplastic Stem Cells
/ metabolism
PC-3 Cells
Prostatic Neoplasms
/ genetics
Recombinant Proteins
/ metabolism
Cancer
Cancer stem-like cells
Lectin
PC-3 cells
rBC2LCN
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
12 07 2019
12 07 2019
Historique:
received:
10
05
2019
accepted:
16
05
2019
pubmed:
28
5
2019
medline:
1
7
2020
entrez:
29
5
2019
Statut:
ppublish
Résumé
The recombinant lectin rBC2LCN is a useful marker for discriminating the undifferentiated status of human induced or embryonic stem cells. Recently, rBC2LCN has also been used for detecting some cancers and niche cells. However, the generality of which types of cells are detected by rBC2LCN is unclear. In this study, we demonstrated the potential of rBC2LCN as a probe for detecting and isolating cancer stem-like cells. Interestingly, flow cytometric analysis of various human cell lines indicated that the human prostate cancer cell line PC-3 consisted of rBC2LCN-positive and -negative subpopulations. Compared with the rBC2LCN-negative subpopulation, the rBC2LCN-positive subpopulation possessed representative features of cancer stem cells and malignancy, such as slow proliferation, increased cell motility, anchorage-independent growth, and drug resistance. The comprehensive expression profiles revealed that the rBC2LCN-positive subpopulation expressed higher levels of cancer stem cell markers. These findings indicate that rBC2LCN is useful for detecting not only pluripotent stem cells but also the cancer stem-like subpopulation of PC-3 cells. Pluripotent and cancer cells with rBC2LCN positivity would be important for future stem cell research.
Identifiants
pubmed: 31133376
pii: S0006-291X(19)30994-5
doi: 10.1016/j.bbrc.2019.05.108
pii:
doi:
Substances chimiques
Biomarkers, Tumor
0
Lectins
0
Molecular Probes
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
176-182Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.