Dual substrate/solvent- roles of water and mixed reaction-diffusion control of β-Galactosidase catalyzed reactions in PEG-induced macromolecular crowding conditions.
Algorithms
Biocatalysis
/ drug effects
Diffusion
Escherichia coli Proteins
/ metabolism
Hydrolysis
/ drug effects
Kinetics
Macromolecular Substances
/ chemistry
Nitrophenylgalactosides
/ chemistry
Polyethylene Glycols
/ metabolism
Solvents
/ chemistry
Thermodynamics
Water
/ chemistry
beta-Galactosidase
/ chemistry
Enzyme diffusion
ONPG
PNPG
Sedimentation velocity
Water structure
macromolecular crowding
β-Galactosidase catalytic activity
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
12 07 2019
12 07 2019
Historique:
received:
29
04
2019
accepted:
10
05
2019
pubmed:
28
5
2019
medline:
1
7
2020
entrez:
29
5
2019
Statut:
ppublish
Résumé
Here we studied the effect of molecular crowding on the hydrolysis of ortho- and para-nitrophenyl-β-D-galactopyranosides (ONPG, PNPG) catalysed by Escherichia coli β-Galactosidase in the presence of 0-35%w/v 6kD polyethyleneglycol (PEG
Identifiants
pubmed: 31133380
pii: S0006-291X(19)30957-X
doi: 10.1016/j.bbrc.2019.05.081
pii:
doi:
Substances chimiques
Escherichia coli Proteins
0
Macromolecular Substances
0
Solvents
0
Water
059QF0KO0R
2-nitrophenylgalactoside
19710-96-4
Nitrophenylgalactosides
28347-45-7
Polyethylene Glycol 6000
30IQX730WE
4-nitrophenylgalactoside
3150-24-1
Polyethylene Glycols
3WJQ0SDW1A
beta-Galactosidase
EC 3.2.1.23
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
190-195Informations de copyright
Copyright © 2019. Published by Elsevier Inc.