The viral protein corona directs viral pathogenesis and amyloid aggregation.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
27 05 2019
Historique:
received: 16 01 2019
accepted: 26 04 2019
entrez: 29 5 2019
pubmed: 28 5 2019
medline: 14 6 2019
Statut: epublish

Résumé

Artificial nanoparticles accumulate a protein corona layer in biological fluids, which significantly influences their bioactivity. As nanosized obligate intracellular parasites, viruses share many biophysical properties with artificial nanoparticles in extracellular environments and here we show that respiratory syncytial virus (RSV) and herpes simplex virus type 1 (HSV-1) accumulate a rich and distinctive protein corona in different biological fluids. Moreover, we show that corona pre-coating differentially affects viral infectivity and immune cell activation. In addition, we demonstrate that viruses bind amyloidogenic peptides in their corona and catalyze amyloid formation via surface-assisted heterogeneous nucleation. Importantly, we show that HSV-1 catalyzes the aggregation of the amyloid β-peptide (Aβ

Identifiants

pubmed: 31133680
doi: 10.1038/s41467-019-10192-2
pii: 10.1038/s41467-019-10192-2
pmc: PMC6536551
doi:

Substances chimiques

Amyloid beta-Peptides 0
Peptide Fragments 0
Protein Aggregates 0
Protein Corona 0
amyloid beta-protein (1-42) 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2331

Subventions

Organisme : Medical Research Council
ID : MR/N024850/1
Pays : United Kingdom

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Auteurs

Kariem Ezzat (K)

Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, 10691, Sweden. kariem.ezzat@su.se.
Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet, Stockholm, 14152, Sweden. kariem.ezzat@su.se.

Maria Pernemalm (M)

Clinical Proteomics Mass Spectrometry, Department of Oncology-Pathology, Science for Life Laboratory and Karolinska Institutet, Stockholm, 17176, Sweden.

Sandra Pålsson (S)

Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, 10691, Sweden.

Thomas C Roberts (TC)

Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX13PT, UK.
Sanford Burnham Prebys Medical Discovery Institute, Development, Aging and Regeneration Program, La Jolla, CA, 92037, USA.

Peter Järver (P)

Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, 10691, Sweden.

Aleksandra Dondalska (A)

Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, 10691, Sweden.

Burcu Bestas (B)

Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet, Stockholm, 14152, Sweden.
Discovery Sciences, R&D Biopharmaceuticals, AstraZeneca, Gothenburg, Sweden.

Michal J Sobkowiak (MJ)

Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, 14186, Sweden.

Bettina Levänen (B)

Unit for Lung and Airway disease, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, 17165, Sweden.

Magnus Sköld (M)

Respiratory Medicine Unit, Department of Medicine, Karolinska Institutet, Stockholm, 17176, Sweden.
Department of Respiratory Medicine and Allergy, Karolinska University Hospital, Stockholm, 17176, Sweden.

Elizabeth A Thompson (EA)

Immunology and Allergy Unit, and Center for Molecular Medicine, Department of Medicine, Karolinska Institutet, Stockholm, 17176, Sweden.

Osama Saher (O)

Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet, Stockholm, 14152, Sweden.
Faculty of Pharmacy, Department of Pharmaceutics and Industrial Pharmacy, Cairo University, Cairo, 11562, Egypt.

Otto K Kari (OK)

Drug Research Program, Faculty of Pharmacy, Division of Pharmaceutical Biosciences, University of Helsinki, Helsinki, 00014, Finland.

Tatu Lajunen (T)

Drug Research Program, Faculty of Pharmacy, Division of Pharmaceutical Biosciences, University of Helsinki, Helsinki, 00014, Finland.

Eva Sverremark Ekström (E)

Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, 10691, Sweden.

Caroline Nilsson (C)

Department of Clinical Science and Education, Södersjukhuset, Karolinska Institutet and Sachs' Children and Youth Hospital, Stockholm, 11883, Sweden.

Yevheniia Ishchenko (Y)

A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, 70211, Finland.

Tarja Malm (T)

A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, 70211, Finland.

Matthew J A Wood (MJA)

Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX13PT, UK.

Ultan F Power (UF)

Centre of Experimental Medicine, Queens' University Belfast, Belfast, BT97BL, UK.

Sergej Masich (S)

Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, 17177, Sweden.

Anders Lindén (A)

Unit for Lung and Airway disease, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, 17165, Sweden.
Department of Respiratory Medicine and Allergy, Karolinska University Hospital, Stockholm, 17176, Sweden.

Johan K Sandberg (JK)

Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, 14186, Sweden.

Janne Lehtiö (J)

Clinical Proteomics Mass Spectrometry, Department of Oncology-Pathology, Science for Life Laboratory and Karolinska Institutet, Stockholm, 17176, Sweden.

Anna-Lena Spetz (AL)

Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, 10691, Sweden. anna-lena.spetz@su.se.

Samir El Andaloussi (S)

Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet, Stockholm, 14152, Sweden.
Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX13PT, UK.
Evox Therapeutics Limited, Oxford Science Park, Oxford, OX44HG, UK.

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Classifications MeSH