Vitrification of stallion sperm using 0.25 ml straws: Effect of volume, concentration and carbohydrates (sucrose/trehalose/raffinose).
Animals
Carbohydrates
/ pharmacology
Cryopreservation
/ methods
Cryoprotective Agents
/ pharmacology
Horses
Male
Raffinose
/ pharmacology
Semen Analysis
/ veterinary
Semen Preservation
/ methods
Sperm Motility
Sucrose
/ pharmacology
Sweetening Agents
/ pharmacology
Trehalose
/ pharmacology
Vitrification
Carbohydrate
Sperm
Stallion
Straw
Vitrification
Volume
Journal
Animal reproduction science
ISSN: 1873-2232
Titre abrégé: Anim Reprod Sci
Pays: Netherlands
ID NLM: 7807205
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
05
02
2019
revised:
14
04
2019
accepted:
20
05
2019
pubmed:
30
5
2019
medline:
13
11
2019
entrez:
30
5
2019
Statut:
ppublish
Résumé
Sperm vitrification is a rapid freezing method in which carbohydrates are used as cryoprotectants. The aim of this study was to determine the optimal volume, concentration and type of carbohydrates for stallion sperm vitrification using 0.25 ml straws in comparison to conventional freezing. Ejaculates (n = 54) were collected from six stallions. For vitrification, straws were filled with different volumes (30, 70, 100 μl), sperm concentrations (50, 100, 200 × 10
Identifiants
pubmed: 31138492
pii: S0378-4320(19)30115-0
doi: 10.1016/j.anireprosci.2019.05.009
pii:
doi:
Substances chimiques
Carbohydrates
0
Cryoprotective Agents
0
Sweetening Agents
0
Sucrose
57-50-1
Trehalose
B8WCK70T7I
Raffinose
N5O3QU595M
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
69-77Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.