Circulating Tumor Cell cluster phenotype allows monitoring response to treatment and predicts survival.
Antineoplastic Agents
/ therapeutic use
Breast Neoplasms
/ blood
Disease Progression
Esophageal Neoplasms
/ blood
Female
Humans
Kaplan-Meier Estimate
Keratins
/ analysis
Lung Neoplasms
/ blood
Neoplastic Cells, Circulating
/ drug effects
Prognosis
Tumor Cells, Cultured
Urinary Bladder Neoplasms
/ blood
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 05 2019
28 05 2019
Historique:
received:
31
07
2018
accepted:
15
05
2019
entrez:
30
5
2019
pubmed:
30
5
2019
medline:
21
10
2020
Statut:
epublish
Résumé
Circulating tumor cells (CTCs) are putative markers of tumor prognosis and may serve to evaluate patient's response to chemotherapy. CTCs are often detected as single cells but infrequently as clusters and are indicative of worse prognosis. In this study, we developed a short-term culture of nucleated blood cells which was applied to blood samples from breast, lung, esophageal and bladder cancer patients. Clusters of different degrees of compactness, classified as very tight, tight and loose were observed across various cancer types. These clusters show variable expression of cytokeratins. Cluster formation from blood samples obtained during the course of chemotherapy was found to be associated with disease progression and shorter overall survival. The short-term cultures offer a robust and highly reliable method for early prediction of treatment response in different cancer types.
Identifiants
pubmed: 31138856
doi: 10.1038/s41598-019-44404-y
pii: 10.1038/s41598-019-44404-y
pmc: PMC6538674
doi:
Substances chimiques
Antineoplastic Agents
0
Keratins
68238-35-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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