Evaluation of urinalysis parameters and antimicrobial susceptibility of uropathogens among out-patients at University of Cape Coast Hospital.


Journal

Ghana medical journal
ISSN: 2616-163X
Titre abrégé: Ghana Med J
Pays: Ghana
ID NLM: 0073210

Informations de publication

Date de publication:
Mar 2019
Historique:
entrez: 30 5 2019
pubmed: 30 5 2019
medline: 18 12 2019
Statut: ppublish

Résumé

Urinary tract infection (UTI) is a major global public health issue. The gold standard for diagnosing UTI is urine culture. This is however labour intensive and time consuming. Many prescribers therefore rely on urinalysis in diagnosing UTI. This study sought to evaluate the performance of some parameters of urinalysis as predictors of urine culture positivity. The common causative agents and their antibiotic susceptibility patterns were also determined. A cross sectional study was carried out at the University of Cape Coast Hospital from July 2017 - December 2017 among out-patients. The performance characteristics of leukocyte esterase (3+) and nitrite reactions were estimated and compared with urine culture. Antimicrobial susceptibility tests were done using disc diffusion technique described by Kirby-Bauer. Prevalence of UTI in this study was 30.0% (64/213). The most prevalent pathogen was E. coli (20, 31.2%), followed by S. saprophyticus (9, 14.1%). Most of the bacteria (52, 94.5%) were sensitive to amikacin, followed by ciprofloxacin (42, 76.3%). The most sensitive (94.4%) of the parameters was pus cells [>5 white blood cells (WBC) per high power field (HPF)] and the least sensitive was the nitrite test (21.0%). The leukocyte esterase test showed the highest accuracy of 91.1%. The study supports the recommendation of the use of oral ciprofloxacin as the first line treatment of uncomplicated UTI by the Ghana Standard Treatment Guidelines (2017). No funding was provided for this study.

Sections du résumé

BACKGROUND BACKGROUND
Urinary tract infection (UTI) is a major global public health issue. The gold standard for diagnosing UTI is urine culture. This is however labour intensive and time consuming. Many prescribers therefore rely on urinalysis in diagnosing UTI. This study sought to evaluate the performance of some parameters of urinalysis as predictors of urine culture positivity. The common causative agents and their antibiotic susceptibility patterns were also determined.
METHODS METHODS
A cross sectional study was carried out at the University of Cape Coast Hospital from July 2017 - December 2017 among out-patients. The performance characteristics of leukocyte esterase (3+) and nitrite reactions were estimated and compared with urine culture. Antimicrobial susceptibility tests were done using disc diffusion technique described by Kirby-Bauer.
RESULTS RESULTS
Prevalence of UTI in this study was 30.0% (64/213). The most prevalent pathogen was E. coli (20, 31.2%), followed by S. saprophyticus (9, 14.1%). Most of the bacteria (52, 94.5%) were sensitive to amikacin, followed by ciprofloxacin (42, 76.3%). The most sensitive (94.4%) of the parameters was pus cells [>5 white blood cells (WBC) per high power field (HPF)] and the least sensitive was the nitrite test (21.0%). The leukocyte esterase test showed the highest accuracy of 91.1%.
CONCLUSION CONCLUSIONS
The study supports the recommendation of the use of oral ciprofloxacin as the first line treatment of uncomplicated UTI by the Ghana Standard Treatment Guidelines (2017).
FUNDING BACKGROUND
No funding was provided for this study.

Identifiants

pubmed: 31138943
doi: 10.4314/gmj.v53i1.7
pii: jGMJ.v53.i1.pg44
pmc: PMC6527828
doi:

Substances chimiques

Anti-Bacterial Agents 0
Ciprofloxacin 5E8K9I0O4U

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

44-51

Déclaration de conflit d'intérêts

Conflict of interest: None declared

Références

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Auteurs

James K Prah (JK)

University of Cape Coast Hospital, University of Cape Coast, Ghana.

Samuel Amoah (S)

University of Cape Coast Hospital, University of Cape Coast, Ghana.

Dickson Wk Ocansey (DW)

University of Cape Coast Hospital, University of Cape Coast, Ghana.

Rudolf Arthur (R)

University of Cape Coast Hospital, University of Cape Coast, Ghana.

Emmanuel Walker (E)

University of Cape Coast Hospital, University of Cape Coast, Ghana.

Dorcas Obiri-Yeboah (D)

Department of Microbiology and Immunology, School of Medical Sciences, College of Health & Allied Sciences, University of Cape Coast, Ghana.

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Classifications MeSH