Mechanistic Basis for Ribosomal Peptide Backbone Modifications.


Journal

ACS central science
ISSN: 2374-7943
Titre abrégé: ACS Cent Sci
Pays: United States
ID NLM: 101660035

Informations de publication

Date de publication:
22 May 2019
Historique:
received: 08 02 2019
entrez: 30 5 2019
pubmed: 30 5 2019
medline: 30 5 2019
Statut: ppublish

Résumé

YcaO enzymes are known to catalyze the ATP-dependent formation of azoline heterocycles, thioamides, and (macro)lactamidines on peptide substrates. These enzymes are found in multiple biosynthetic pathways, including those for several different classes of ribosomally synthesized and post-translationally modified peptides (RiPPs). However, there are major knowledge gaps in the mechanistic and structural underpinnings that govern each of the known YcaO-mediated modifications. Here, we present the first structure of any YcaO enzyme bound to its peptide substrate in the active site, specifically that from

Identifiants

pubmed: 31139720
doi: 10.1021/acscentsci.9b00124
pmc: PMC6535971
doi:

Types de publication

Journal Article

Langues

eng

Pagination

842-851

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM097142
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM131347
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR027109
Pays : United States

Déclaration de conflit d'intérêts

The authors declare no competing financial interest.

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Auteurs

Shi-Hui Dong (SH)

Department of Biochemistry, Carl R. Woese Institute for Genomic Biology, Department of Microbiology, Department of Chemistry, and Center for Biophysics and Quantitative Biology, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, United States.

Andi Liu (A)

Department of Biochemistry, Carl R. Woese Institute for Genomic Biology, Department of Microbiology, Department of Chemistry, and Center for Biophysics and Quantitative Biology, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, United States.

Nilkamal Mahanta (N)

Department of Biochemistry, Carl R. Woese Institute for Genomic Biology, Department of Microbiology, Department of Chemistry, and Center for Biophysics and Quantitative Biology, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, United States.

Douglas A Mitchell (DA)

Department of Biochemistry, Carl R. Woese Institute for Genomic Biology, Department of Microbiology, Department of Chemistry, and Center for Biophysics and Quantitative Biology, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, United States.

Satish K Nair (SK)

Department of Biochemistry, Carl R. Woese Institute for Genomic Biology, Department of Microbiology, Department of Chemistry, and Center for Biophysics and Quantitative Biology, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, United States.

Classifications MeSH